Re: marking tumor margins

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From:Mick Rentsch <> (by way of histonet)
To:histonet <>
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Dear Margaret,
Eosin has a habit of leaching out during processing. We often use 4% Alcian
Blue Aq. with a crystal of thymol (You do not filter it.) esp. for breast
biospies. The preparation may become glutinous with time but it is still
effective and you can dab it on with either a cotton bud or cotton ball if
you have a whole mastectomy.
Regars MIke Rentsch (Downunder)
-----Original Message-----
From: Margaret Gondo <>
To: <>
Date: Tuesday, 6 October 1998 2:06
Subject: marking tumor margins

>Hi 'Netters-
>I've never had to do this so I am going to ask all of you.  What do dye you
>use to mark tumor margins?  I've often received blocks where the tissue
>looked like it had been dipped in alcian blue when it was grossed in.  I've
>also heard of eosin being used.  I would prefer to have something darker
>than eosin.  What I am planning on doing is resecting some mouse tumors,
>dipping them in something so I know which end is which, fixing in the PLP
>fixative (periodate-lysine-paraformaldehyde) and then processing to
>Any suggestions?
>Thanks alot!

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