Re: marking tumor margins

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From:Jeff Silverman <peptolab@hamptons.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Dear Margaret; CMS-Fisher Scientific sells India Ink.  We dab it on with a
cotton-tip applicator and dip the freshly inked specimen in acetone to fix
the ink.  The acetone probably won't effect your studies but be careful.
Jeff Silverman
peptolab@hamptons.com

----------
> From: Margaret Gondo <gondom@genemedicine.com>
> To: histonet@pathology.swmed.edu
> Subject: marking tumor margins
> Date: Monday, October 05, 1998 10:01 AM
>
> Hi 'Netters-
>
> I've never had to do this so I am going to ask all of you.  What do dye
you
> use to mark tumor margins?  I've often received blocks where the tissue
> looked like it had been dipped in alcian blue when it was grossed in.
I've
> also heard of eosin being used.  I would prefer to have something darker
> than eosin.  What I am planning on doing is resecting some mouse tumors,
> dipping them in something so I know which end is which, fixing in the PLP
> fixative (periodate-lysine-paraformaldehyde) and then processing to
> paraffin.
>
>
> Any suggestions?
>
> Thanks alot!
>
> Margaret
>
>




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