Re: marking tumor margins

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From:Jeff Silverman <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Dear Margaret; CMS-Fisher Scientific sells India Ink.  We dab it on with a
cotton-tip applicator and dip the freshly inked specimen in acetone to fix
the ink.  The acetone probably won't effect your studies but be careful.
Jeff Silverman

> From: Margaret Gondo <>
> To:
> Subject: marking tumor margins
> Date: Monday, October 05, 1998 10:01 AM
> Hi 'Netters-
> I've never had to do this so I am going to ask all of you.  What do dye
> use to mark tumor margins?  I've often received blocks where the tissue
> looked like it had been dipped in alcian blue when it was grossed in.
> also heard of eosin being used.  I would prefer to have something darker
> than eosin.  What I am planning on doing is resecting some mouse tumors,
> dipping them in something so I know which end is which, fixing in the PLP
> fixative (periodate-lysine-paraformaldehyde) and then processing to
> paraffin.
> Any suggestions?
> Thanks alot!
> Margaret

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