At 10:35 AM 10/8/98 +0000, Cathy Fragiskatos wrote:
>
>    Dear histonetters,
>
>    I was wondering if anyone out there has run a study on various
>fixation protocols for cell blocks.  If so, I have a few questions for
>these individuals:
>
>1-  What was the best overall fixation protocol for cell morphology?
my guess would be 10% neutral buffered formalin and paraffin embedding

>2-  What was the best fixation protocol for CD markers? (particularly
>for B and T cell markers)

for this it's best to have frozen sections fixed with acetone @ -20 for at
least a couple of minutes (some have told me 10 min, some have said as
little as 30 sec) If you must do paraffins and want to use CD3 use the Dako
Ab.
>
>    Any information forwarded would be greatly appreciated.
>
>    Best Regards,
>
>    Cathy Fragiskatos
>    IHC Laboratory
>    Mc Gill University/ Royal Victoria Hospital
>    Montreal, Quebec.
>
>    e-mail: fragiska@pathology.lan.mcgill.ca
>
>
>
>
Marcia Bentz
Lab Specialist Senior
UVA
Dept Internal Medicine/GI
804-243-6895