Re: Special stains

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From:Mick Rentsch <ausbio@nex.com.au> (by way of histonet)
To:histonet <histonet@magicnet.net>
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You could try Richard Hazelton Bowral Hospital NSW. Aust. you can email him
on histotek@hinet.com.au . Richard is currently working on a CD ROM for
techniques esp. special stains and aims to include as many photographs as
possible particularly at different stages of differentiation etc.
Regards MIke Rentsch
-----Original Message-----
From: swansong <swansong@videotron.ca>
To: HistoNet Server <HistoNet@Pathology.swmed.edu>
Date: Monday, 12 October 1998 1:39
Subject: Re: Special stains


>Hello everybody!!, Well then let me begin!
>I'm in dire need of pictures demonstrating special stains such as Masson
>Fontana, PAMS,GAF, PAS, Verhoeff Van Gieson,HPS, WVG,GRT,GMS.
>Could anyone please direct me to the proper websites which will contain
>these pictures,
>Thank you for your time.
>-----Original Message-----
>From: HistoNet Server <HistoNet@Pathology.swmed.edu>
>To: HistoNet Server <HistoNet@pathology.swmed.edu>
>Date: Sunday, October 11, 1998 1:03 AM
>Subject: Daily Digest
>
>
>>
>>----------------------------------------------------------------------
>>
>>Date: 10 Oct 1998 00:48:05 -0500
>>From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
>>Subject: Re: Your failed attempted histonet defection
>>
>>
>>On Fri, 9 Oct 1998, C***y H*******n wrote:
>>
>>> unsubscribe
>>
>> The usual slip is to spell it wrongly.
>> You got it right but you put it in the wrong place.
>>
>> The sacred word UNSUBSCRIBE has to go in the Subject line
>> of your email. Be careful not to write unsuscribe or
>> unscribe or unsusribe, or the other things we see so
>> often.
>>
>> It's an awful word to type, and just one wrong letter
>> results in the whole group knowing all about your bungled
>> attempt at desertion!
>>                        John Kiernan
>>                        London, Canada.
>>
>>
>>
>>
>>----------------------------------------------------------------------
>>
>>Date: 10 Oct 1998 01:00:08 -0500
>>From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
>>Subject: Amyloid can be obscure. Re: congo red
>>
>>On Fri, 9 Oct 1998, Bryan Llewellyn wrote:
>>
>>> I have run into this on a few occasions.  It is in the nature of amyloid
>to
>>> be variable in staining.  If you really must prove its presence, then
cut
>>> some of the tissue out of the block, dewax it and send it for EM.
>Although
>>> the ultrastructural morphology will be the pits, the very characteristic
>>> structure of amyloid is retained.  We did this on a case a few years
ago,
>>> when the only stain that demonstrated it was thioflavin T.  It did turn
>out
>>> to be amyloid.
>>                 Bryan Llewellyn
>>
>>  This is a real HistoNet gem!
>>                               John Kiernan
>>                               London, further E. in Canada.
>>
>>
>>
>>
>>----------------------------------------------------------------------
>>
>>Date: 10 Oct 1998 07:25:49 -0500
>>From: "Yvan Lindekens" <yvan.lindekens@skynet.be>
>>Subject: Re: "xylene AND alcohol substitude" & "rabbit spleen in
>formaldehyde
>>37%"
>>
>>hi all,
>>
>>ETOH and xylene can be ommitted in the embedding process (paraffin) by the
>>use of 2-propanol. This calls for dehydration in 2-propanol at room
>>temperature, 2-propanol at 50#161#C, 2-propanol-paraplast mixture (1/1
v/v)
>at
>>50#161#C and finally, normal embedding in Paraplast.
>>
>>I use this method all the time (histology and plant anatomy); It works
very
>>well... I have tried it with several other paraffin's (Paraplast-plus,
>>"paraffin fur die histologie", Merck and even with a mixture of household
>>paraffin, mp. 52#161#C  and purified biewax (95/5 w/w). The results were
>OK,
>>sometimes even better compared to the results obtained by "classical
>>techniques". Cutting artefacts are far less, due to the abcense of the
>>hardening effect of xylene (benzene, toluene)...
>>(sections 4-12um, RT= 16#161#C, with manualy operated rotary microtome and
>>c-type knive: as an amateur, I don't believe in disposable blades:
grinding
>>and polishing knives is part of the fun).
>>
>>One drawback: dehydration trough 2-propanol is slower than dehydration
>>trough ETOH...
>>
>>The method is described in Bock, P.: "Romeis' Mikroskopische Technik",
>>1989, Urban&Schwarzenberg, ISBN: 3-541-11227-1.
>>
>>It's a modification of an old technique (Dietrich, 1929; Doxtader, 1948)
>>and I think (but I'm not sure) that even Bolles Lee mentioned it in one of
>>the first editions of "The Microtomist's vademecum"... (If my dear friend
x
>>would be so kind to, finally, return my copy? Thanks).
>>___________________________________________________________
>>There was a messages about rabbit spleen, "par accident" fixed in
>>formaldehyde 37% (Mrs. Mary Kay Olson). Embarrasing (know it, been
>>there...).
>>
>>Perhaps you could give the 2-propanol-paraplast technique a try instead of
>>a regular ETOH-xylene-parrafin run: tissue hardening will be less and
>>perhaps it will be possible to cut usable sections... Cutting on a sliding
>>microtome (instead of a rotary-) could help too...
>>
>>Mr. Paul Millikin said in his response: "...spleens are VERY hard to fix
>>WELL... ...They tend to be so full of RBC that they behave like a thrombus
>>or an embolus... When simply thrown intact into an immersion fixative, the
>>outer, subcapsular 2-3 mm tends to fix, but the rest is usually rotten...
>>... We've been looking at rotten, autolyzed tissue for too long...
>>...Slicing (breadloafing) the specimen every 2-3 mm before immersion
causes
>>considerable improvement, and should be a must for everyone interested in
>>spleens".
>>
>>This is certainly true... I always slice spleen (and other hard to fix
>>tissues as lung etc...) in slices about 2-3 mm thick. This can easily be
>>done with a sharp razor, a long razor blade or a large dissecting knive
(no
>>scalpel: the blade is too "short"...) and without using pressure.
>>Even then, adequate fixation isn't always guaranteed when using NBF... I
>>believe it's better to fix in an alcoholic Bouin formulation
>>(Duboscq-Brasil, Lang, Rossman...)(depending on the purpose of the
>>preparations).
>>I know that there are conflicting opinions regarding the fixation of
spleen
>>in Bouin, but if I have to choose between techniques that "are not
generaly
>>accepted" and making slides of "rotten, autolyzed tissue"...
>>
>>Yvan Lindekens.
>>
>>
>>
>>----------------------------------------------------------------------
>>
>>Date: 10 Oct 1998 10:42:32 -0500
>>From: "Alexander Nader" <anader@teleweb.at>
>>Subject: Re: Mercury poisoning tissue stain?
>>
>>On Thu, 08 Oct 1998 19:00:13 -0700, A. Mark Briones wrote:
>>
>>>Has anyone been able to demonstrate tissue deposition in acute, possibly
>>>chronic mercury poisoning cases? Does anyone have a good tissue stain for
>>>mercury deposits, our pathologist would like to demonstrate deposition if
>it
>>>can be stained? Kidney? Liver? Brain?  Are there any tissues in anyone's
>>>"banks" that has demonstrated positive mercury staining so I could
>validate
>>>any finding on this unfortunate case?  Thanks.
>>
>>what about back-scattered electron imgaging? We do this as element
analysis
>to
>>show the content of Ca,
>>Fluor in bone. I' ll ask my REM technician on monday if it is possible
with
>>mercury too.
>>
>>
>>
>>
>> Dr. Alexander Nader
>>            Rossauer Laende 35a/32
>>      A-1090 WIEN
>>
>>    anader@teleweb.at
>>
>>
>>
>>----------------------------------------------------------------------
>>
>>Date: 10 Oct 1998 13:17:59 -0500
>>From: Priscilla <pdelvent@wyoming.com>
>>Subject: Charges for multiple tissues
>>
>>Hi everyone in Histo Land--We are having a discussion about making a
>>special charge for specimens that arrive in multiple for processing.
>>Things like more than one skin specimen, colon bxs, prostate needle bxs,
>>etc.  In the past I have always had a charge of XXX multiple where I lower
>>the price of the single specimen and then times it the number of specimens
>>that have been removed and called it skin multiple or prostate multiple or
>>colon multiple.  These would all be submitted in separate containers and
>>thusly separate cassettes.  The colon bxs especially can amount to more
>>dollars charge than to remove the whole colon if the single specimen
charge
>>would be multiplied times the number of sites bxs.
>>
>>How do you handle these charges?
>>
>>Also in researching these charges by calling other hospitals, I came
across
>>another interesting method of charging.  I have found where even if a
>>bilateral specimen such as vas deferens or fallopian tubes were submitted
>>in the same container  (Yes, I too, hope that there is a suture on the
>>right sided specimen to denote which it is) that the charge was x two even
>>though it was submitted in the same container.  Interestingly enough, I
>>have always submitted only one charge for a bilateral fallopian tube or
vas
>>deferens, even if they were submitted in separate containers (which I hope
>>they were)
>>
>>Also in the case of uterus, fallopian tubes and ovaries, I found where
>>there were three charges even though the specimen was submitted in one
>>container.  For Tonsils and Adenoids, I found places charging 3 charges
for
>>this type of specimen.  I have always charged only one charge for either
>>both tonsils or tonsils and adenoids.
>>
>>Either I have been very stupid or just not up to date on charging
>>practices.  I'm sure our patients hope that I don't become too informed as
>>changing the way I've been charging will make a big impact on our
customers
>>bills.
>>
>>Does any one know of rules for this?  I always thought that anything sent
>>down in a separate container needed a separate charge, except in the case
>>of bilateral specimens such as those mentioned above, and I thought that
>>T&A's were submitted separately just so the specimen could be identified
as
>>to the proper side,  I also thought that UTO's were considered one
>>specimen.  I can see where a uterus sent separately from the ovaries could
>>justify a separate charge, especially if one ovary was possibly neoplastic
>>or the uterus was and the ovaries benign.  Of course we don't know until
>>the microscopic, do we?
>>
>>I look forward to being enlightened by this group.  Please be kind to me!!
>>I'm usually on top of these things, but coding workshops usually are for
>>the total billing and I don't get sent to them if they don't concentrate
on
>>just Pathology.
>>
>>Priscilla in Central Wyoming
>>
>>
>>----------------------------------------------------------------------
>>
>>Date: 10 Oct 1998 16:21:17 -0500
>>From: Michelle McCafferty <mmccaff@mbl.edu>
>>Subject: Thank you
>>
>>
>> Thank you to every one who explained what the Red Green Show is
>>all about.  Boston area PBS doesn't have it listed on Saturday night so I
>>guess I'll have to check the other nights of the week.  Perhaps Boston
>>isn't broadcasting the show at all!  Sounds like that would be a pity.
>>
>> Michelle
>>
>>
>>
>>Michelle McCafferty
>>Marine Biological Laboratory
>>7 MBL Street
>>Woods Hole, MA 02543-1015
>>
>>
>>
>>
>>----------------------------------------------------------------------
>>
>>Date: 10 Oct 1998 18:01:00 -0500
>>From: swansong <swansong@videotron.ca>
>>Subject: Re: subscribe digest
>>
>>
>>- -----Original Message-----
>>From: HistoNet Server <HistoNet@Pathology.swmed.edu>
>>To: swansong <swansong@videotron.ca>
>>Date: Friday, October 09, 1998 7:42 PM
>>
>>
>>>Please do not send ENCLOSURES!!!!!!
>>>
>>
>>
>>
>>Here are the messages received yesterday!
>>
>
>




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