Re: Re[2]: Sterilization Solution

<< Previous Message | Next Message >>
From:Barry Rittman <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Re sterilization,
			I believe that the only options are
1.	steam or dry heat sterilizing
2.	glutaraldehyde
3.	chlorhexidine
4.	flaming

For the specific situation that you are talking about, I would suggest
steam or heat sterilizing as there are a variety of animals from different
situations, and because of this you may have some sporing organisms.
Glutaraldehyde is an option providing that first any debris is removed from
the instruments before placing in the glutaraldehyde and that the
instruments are rinsed off with sterile distilled water or saline before
using. If sporing organisms are a problem then the long time in
glutaraldehye necessary to kill spores(6 hrs or so) is  not practical.
Chlorhexidine is also bactericide but again takes a long time to kill
sporing organisms.

Flaming is also an option.

Alcohol (70%) is regarded as a disinfectant but is not very useful unless
debris is first removed as it will cause blood to become very firmly attached.

If you are considering doing an operation on several small lab animals such
as mice then the chances of sporing orgnaisms is not very great and alcohol
can be used between instruments providing that it is removed either by
flaming or evaporation before use. Use of instruments which contain traces
of alcohol can compromise the anesthesia (at least of small rodents, not
sure if this applies to larger animals).

At 10:40 AM 10/1/98 -0400, you wrote:
>     For iodophor solution, try your local homebrew store.  A good cheap
>     alternative is 10% hypochlorite bleach, although this can corrode some
>     steels.  The Iodophor solution will discolor some plastics (my
>     polyethylene primary fermenter being one)
>     Regards
>     Simon
>______________________________ Reply Separator
>Subject: Re: Sterilization Solution
>Author: at Internet
>Date:    9/30/98 7:45 PM
>Dear Joe,
>because of the high risk of sensitisation with Cidex and other
>Glutaradlehyde products, I would advise most strongly against these types of
>products despite their effeciacy. Most appropriate would either be Water or
>alcoholic based chlorhexidine solutions (also known as Cetrimide TM ICI);
>these are available pre-formulated with one of these being extensively used
>in the Dairy Industry as an udder wash incorporating a detergent. Iodophor
>compounds would not be an appropriate choice for instruments. Whatever is
>used it is imperative that the instruments be scrupulously clean before
>sterilisation. As a starting point for a supplier I would suggest either
>Baxter Healthcare or Johnson&Johnson.
>Regards Mike Rentsch (Spanner)
>-----Original Message-----
>From: Saby, Joseph <>
>To: '' <>
>Date: Thursday, 1 October 1998 3:03
>Subject: Sterilization Solution
>>A colleague has asked me to post a question for a friend of hers who
>>works in a private vet's office.
>>Specifically, she wanted to know if what would be the best sterilization
>>solution for surgical instruments.
>>Thanks in advance for your information.
>>Joe Saby, BA HT
>>Parke-Davis, Ann Arbor, MI

<< Previous Message | Next Message >>