Re: Mercury poisoning tissue stain?
<< Previous Message | Next Message >>
From: | oshel@terracom.net (Philip Oshel) (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Back-scattered imaging is not element-specific. It just returns a stronger
signal from heavier (higher atomic-number) elements because they're bigger
and so "reflect" more primary beam electrons.
Since Hg is much heavier than most of the atoms in tissues, you can expect
a brighter signal, but you can't claim that this signal is from Hg without
other supporting evidence (e.g. x-ray or chemical).
Futher, back-scattered imaging also has less (or much less) spatial
resolution than normal secondary-electron imaging, so it may not be
adequate if you need to state that the Hg "is in these cells, but not those
cells".
Phil
>
>>Has anyone been able to demonstrate tissue deposition in acute, possibly
>>chronic mercury poisoning cases? Does anyone have a good tissue stain for
>>mercury deposits, our pathologist would like to demonstrate deposition if it
>>can be stained? Kidney? Liver? Brain? Are there any tissues in anyone's
>>"banks" that has demonstrated positive mercury staining so I could validate
>>any finding on this unfortunate case? Thanks.
>
>what about back-scattered electron imgaging? We do this as element
>analysis to show the content of Ca,
>Fluor in bone. I' ll ask my REM technician on monday if it is possible
>with mercury too.
> Dr. Alexander Nader
> Rossauer Laende 35a/32
> A-1090 WIEN
>
> anader@teleweb.at
****be famous! send in a tech tip or question***
Philip Oshel
Technical Editor, Microscopy Today
PO Box 620068
Middleton, WI 53562
(608) 833-2885
oshel@terracom.net
or poshel@hotmail.com
<< Previous Message | Next Message >>