Re: Kappa/Lambda

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From:"Hagerty, Marjorie A." <> (by way of histonet)
To:histonet <>
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Immuno for the Kappa/Lambda. As you stated very aptly, I did not use a
completely accurate control as it was not decalcified. I used declere for
antigen retrieval. Do you suggest I try sat. NaOH? If so for how long?
From: John Wojcieszyn
To: mhagerty
Subject: Re: Kappa/Lambda
Date: Monday, October 05, 1998 12:10PM

Did you do immuno or ISH for the kappa / lambda???? With theses specialy
treated tissues, i.e. decal, the control must have the same treatment to
be accurate . I always match the tissue treatment to the case and here at
the vet school I always match the species as well. Acid treatment will ,
in a sense, be a second fixation and change antigent retrieval times. Did
you use sat. NaOH for retrieval???

john w. wojcieszyn Ph.D.

>>> "Hagerty, Marjorie A." <> 10/05 2:51 PM >>>

Hi fellow listees,
Would decalcification affect Kappa/Lambda staining?  Controls came out
but no staining on patient which pathologist insists should have
The stains were done on a bm core bx which had been decalcified in RDO.
T/B cell stains on the same block came out beautiful. Any ideas/thoughts

from you experts? I am just a user!?!

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