If I had to decalcify a speciment that I later would need to do IHC on, I
would not use any HCl based demineralizer.  I would suspect that that is
your culprit.


At 12:51 PM 10/5/98 -0700, Hagerty, Marjorie A. wrote:
>
>Hi fellow listees,
>Would decalcification affect Kappa/Lambda staining?  Controls came out well
>but no staining on patient which pathologist insists should have stained.
>The stains were done on a bm core bx which had been decalcified in RDO. The
>T/B cell stains on the same block came out beautiful. Any ideas/thoughts
>from you experts? I am just a user!?!
>Thanks
>Marg
>EMC
>
>