Re: HELP: smooth muscle actin antibody

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Beverly wrote:
>Looking for experts on the use of smooth muscle actin antibody, in
>particular clone 1A4, on small vessels in lung tissue, especially those
>who have done morphometric analysis of smooth muscle cells. Help with
>protocols, fixation issues, epitope retrieval, etc.
Dear Bev,
I have used this antibody on paraffin (LM) and Lowicryl HM20 (LM and EM)
embedded tissue. Tissue was fixed in 4% paraformaldehyde in PBS for 2hrs.
No antigen retrieval. Dilution 1/200-1/400. Incubation time: 1 hr.
Detection:  two-step immunogold/silver staining with ultra-small gold.
Regards, Peter

Peter van de Plas
Costerweg 5
6702 AA Wageningen
The Netherlands
phone: (31)-317-497676
fax: (31)-317-415955

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