Re: DAB colour

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From:"Peter A. Takes" <> (by way of histonet)
To:histonet <>
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Dr. Grabsch:

What you are observing is some of the metachromatic characteristics of DAB.
A very common phenomenon.  It can be a function of concentration, incubation
time, etc.  There are publications on this issue, but the exact reference
citations are not immediately available to me at this time.

Peter A. Takes, Ph.D., RAC
Director, Clinical & Regulatory Affairs
Ph. 1-314-615-6964; Pager: 841-9351

Heike Grabsch wrote:

> We use DAB as chromogen in ICC-studies. At the moment I am wondering why
> we get do different shades of coulours from almost black, pale very nasty
> looking dark brown to bright orange-brown.
> I do not like it, I would like to have the same nice brilliant colour
> every day. Are there any ideas about may be the chemical process behind
> this. Is it a matter of concentration (which is the same always) or
> temperature or what.
> Any comments and help to get a reproducible nice brilliant colour are
> highly appreciated.
> Dr. Heike Grabsch, Germany


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