Re: Cy2 fading
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From: | Achim.Schott@bio.uni-giessen.de (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
> Date: Thu, 1 Oct 1998 12:23:04 -0400 (EDT)
> From: Tamara Howard <howard@cshl.org>
> To: Achim.Schott@bio.uni-giessen.de
> Subject: Cy2 fading
> Have you looked at the Jackson Lab's website (I'm not sure of the
> address, but I'm sure a search would find it)? They had some info about Cy
> dye fading/mounting...There seem to be problems with cleavage of the dye
> itself when samples are stored for long periods in certain mounting media.
>
>
> What are you using as mounting media? We use glycerol + 1mg/ml
> para-phenylenediamine (pH above 8.0) and the fading hasn't been too
> horrible with only short-term storage.
>
> Tamara Howard
> CSHL
>
> In the information sheet sent with the antibody, the company informs you
>that the
mounting media containing phenylenediamine may cause some trouble for
long-term storage. I use for Cy3 where these problems did not appear
Vectashield as mounting solution. In the specimen stained with Cy2
and "treated" with Vectashield the fading was also too much ( but it
contains phenylenediamine). I think I will try Mowiol or Dabco as
mounting media.
Thanks for your and everybodies advice
Achim Schott
>
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