Re: CD5

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From:Alexander Nader <anader@teleweb.at> (by way of histonet)
To:histonet <histonet@magicnet.net>
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On Fri, 02 Oct 1998 15:41:43 +0000 (GMT), Ms Louise Taylor wrote:

>they have closed down......! I need to find out if Mantle cell
>lymphomas will be detected using this particular antibody. We are
>using the NCL-CD5 4C7 from Novocastra at the moment . Any information
>i

Novocastras AB works fine for us, but you have to be careful in the Antigen
retrieval step. We use 20 min
steamer followed by 30 min cooling. Also this antibody for unknown reason
does have problems (same as
CD2 and CD 7 from Novocastra) with DAKO's new enhancement kit. We therefore
use Vectastain.


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