Rat Macrophage - Staining Problems
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From: | Dave Tacha <dtacha@ncal.verio.com> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
We been using a "mouse anti-rat macrophage" from Pharmagen. We
incubated the rat tissue (lung) for 1 hour at 10 and 20 micrograms per
ml. I also pretreated the tissue with and without antigen retrieval. I
used Pharmagen's goat anti-mouse with minimal cross-reactivity to rat.
However, we are not getting any staining.
Could someone help me with a procedure, or technical hint; or another
perhaps another rat macrophage antibody that works in formalin-fixed
paraffin embedded tissues?
My main concern is that the secondary antibody may not recognize the
primary antibody.
Has anyone ever heard of MOMA?
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