RE: marking tumor margins

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From:"Weems, Joyce" <> (by way of histonet)
To:histonet <>
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Alligence carries TBS tissue dyes, p557 in the catalog. Also Davidson
has a good tissue dye system, but I don't have the Co info. J

>Sent: 	Monday, October 05, 1998 12:14 PM
>Subject: 	Re: marking tumor margins
>We use India Ink and a very fine paint brush.  I have also seen some posts
>on the 'net regarding tatoo inks and someone mentioned that there is a
>that sells special inks for Histology.  I foget what the name of the Co. is
>though :-(
>-- Begin original message --
>Hi 'Netters-
>I've never had to do this so I am going to ask all of you.  What do dye you
>use to mark tumor margins?  I've often received blocks where the tissue
>looked like it had been dipped in alcian blue when it was grossed in.  I've
>also heard of eosin being used.  I would prefer to have something darker
>than eosin.  What I am planning on doing is resecting some mouse tumors,
>dipping them in something so I know which end is which, fixing in the PLP
>fixative (periodate-lysine-paraformaldehyde) and then processing to
>Any suggestions?
>Thanks alot!
>-- End original message --
>Robert Schoonhoven
>Laboratory of Molecular Carcinogenesis and Mutagenesis
>Dept. of Environmental Sciences and Engineering
>University of North Carolina
>Chapel Hill, NC 27599
>office 919-966-6343
>   Lab 919-966-6140
>   Fax 919-966-6123
>**I'm willing to make the mistakes if someone else is willing to learn from

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