We have found B-5 fixative is very good for CD-15 and CD-30 antibodies.
 Don Luke, HT (ASCP)
SOUTH GEORGIA MEDICAL CENTER
DEPARTMENT OF PATHOLOGY
2501 N. Patterson St
VALDOSTA, GA 31603
PHONE (912) 259-4830



>----------
>From: 	Marcia Bentz[SMTP:mb7x@virginia.edu]
>Sent: 	Thursday, October 08, 1998 1:04 PM
>To: 	Cathy Fragiskatos; histonet@pathology.swmed.edu
>Subject: 	Re: cell block fixation
>
>At 10:35 AM 10/8/98 +0000, Cathy Fragiskatos wrote:
>>
>>    Dear histonetters,
>>
>>    I was wondering if anyone out there has run a study on various
>>fixation protocols for cell blocks.  If so, I have a few questions for
>>these individuals:
>>
>>1-  What was the best overall fixation protocol for cell morphology?
>my guess would be 10% neutral buffered formalin and paraffin embedding
>
>>2-  What was the best fixation protocol for CD markers? (particularly
>>for B and T cell markers)
>
>for this it's best to have frozen sections fixed with acetone @ -20 for at
>least a couple of minutes (some have told me 10 min, some have said as
>little as 30 sec) If you must do paraffins and want to use CD3 use the Dako
>Ab.
>>
>>    Any information forwarded would be greatly appreciated.
>>
>>    Best Regards,
>>
>>    Cathy Fragiskatos
>>    IHC Laboratory
>>    Mc Gill University/ Royal Victoria Hospital
>>    Montreal, Quebec.
>>
>>    e-mail: fragiska@pathology.lan.mcgill.ca
>>
>>
>>
>>
>Marcia Bentz
>Lab Specialist Senior
>UVA
>Dept Internal Medicine/GI
>804-243-6895
>
>
>
>
>