RE: Kappa/Lambda

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From:"Sebree Linda A." <la.sebree@hosp.wisc.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Hi Marjorie,

We routinely stain for K and L on B5 fixed BM BXs using the same
protocol as for soft, non-decaled tissue.  Our BMs are decaled in RDO.

Linda Sebree, HT
University of Wisconsin Hospital & Clinics
Department of Laboratory Medicine
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI  53792-2472

Phone:  (608)265-6596
FAX:  (608)263-1568

> -----Original Message-----
> From:	Hagerty, Marjorie A. [SMTP:mhagerty@emc.org]
> Sent:	Monday, October 05, 1998 2:51 PM
> To:	Histonet
> Subject:	Kappa/Lambda
>
>
> Hi fellow listees,
> Would decalcification affect Kappa/Lambda staining?  Controls came out
> well
> but no staining on patient which pathologist insists should have
> stained.
> The stains were done on a bm core bx which had been decalcified in
> RDO. The
> T/B cell stains on the same block came out beautiful. Any
> ideas/thoughts
> from you experts? I am just a user!?!
> Thanks
> Marg
> EMC




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