Have you changed your buffers lately? Do you use a different buffer for
the automated stainer? Could something have been made up wrong? Could
there be some reaction between your substrate buffer and your dilution
buffer? Are your antibodies made up in a buffer different than your wash
buffer?

Is your substrate source different than before you had problems?

Try going back to when you didn't have any problem and determine what
has changed since then.

Tim Morken, B.S., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

FAX:  (404)639-3043


----Original Message Follows----
Date: Tue, 06 Oct 1998 08:08:28 -0400
From: "Coskran, Timothy M" <timothy_m_coskran@groton.pfizer.com>
To: "'Histonet@pathology.swmed.edu'" <Histonet@pathology.swmed.edu>

Hello,

Does anyone know of a source for I-CAM that will work on paraffin and be
reactive with rat tissue?

We've been having an annoying problem pop up with the last few immuno
runs.
Our slides have "developed" a brown square that covers the tissue
section.
The square is outlined where the pap pen line would have been before
being
dissolved.  The funny thing is that it happens on all the slides except
our
negative control.  This problem seems to only happen on our
immunostainer
and has been showing up with a number of different antibodies.  It
happens
occasionally on runs done on the bench but few and far between and when
it
does occur it usually is with one to two slides max. Any ideas?

Tim







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