Hi Cheryl and Histonetters,

Very early in my career, we did this very same stain (that we called
HBFP) mostly on autopsy cases to rule out myocardial infarction.  But it
was clear to me right away that one could determine the outcome of the
stain be how and how long one differentiated the slides.  In essence,
you could make it turn out positive or negative!

I talked to the person(s) at Mayo that developed the stain and they were
quite closed mouthed about the whole thing and ultimately told me they
were not standing behind their stain anymore for the very reason I
mentioned.

Now, this was in the mid 70's so I don't know what's become of this
stain since though I don't know anyone these days doing it.

Cheryl, I don't know what your original question was, but I threw my 2
cents in anyway.

Linda Sebree, HT
University of Wisconsin Hospital & Clinics
Department of Laboratory Medicine
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI  53792-2472

Phone:  (608)265-6596
FAX:  (608)263-1568

> -----Original Message-----
> From:	Cheryl Crowder [SMTP:crowder@vt8200.vetmed.lsu.edu]
> Sent:	Wednesday, October 07, 1998 7:36 AM
> To:	HistoNet@pathology.swmed.edu
> Subject:	Early Heart failure
>
> Michael - Years ago, we did a Hematoxylin - basic fuchsin - picric
> acid
> stain for early myocardial ischemia.  This may be helpful for your
> panel of
> heart stains.  It was in the Mayo Clinic Proceeding, Vol. 46, May
> 1971, p.
> 319-327.  Results:  "After decolorization, the normal myocardium lost
> its
> affinity for basic fuchsin dye and was stained uniformly light brown
> by the
> picric acid.  The myofibrils and cross-striations were clearly visible
> and
> the nuclei retained the purple hue of hematoxylin".
> 	If you can't get a copy of the article, contact me personally
> and I will
> send a copy to you.  Cheryl
>