Jan,

In response to your inquiry about vendors that monitor the histonet... I am
with NEN Life Sciences.  We unfortunately were not able to make this years
NSH meeting, but would like to be kept up to date so that we will be able to
be there for next years meeting.  Our TSA product line is a revolutionary
product for IHC and ISH methodologies.

My contact info is:

Yoshika Nayak
NEN Life Sciences
ph: (617) 350-9517
email: nayaky@nenlifesci.com

I look forward to hearing from you.

> -----Original Message-----
> From:	HistoNet Server [SMTP:HistoNet@Pathology.swmed.edu]
> Sent:	Wednesday, September 30, 1998 2:02 AM
> To:	HistoNet Server
> Subject:	Daily Digest
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 02:16:14 -0500
> From: Gitte Sjorslev <Gitte.Sjorslev@dako.dk>
> Subject: Microtomes
>
> Hi Histonetters
> I am also looking for a new microtome (rotation) and has been in contact
> with several companies. Does anybody know the quality of microtomes from
> the company RMC?
> Best regards Gitte
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 05:31:05 -0500
> From: Judy Adams <histolab@yahoo.com>
> Subject: Texas labs
>
> I am a histologist, and I am relocating to Texas, the DFW area at the
> end of the year. I would like some names of labs in that area that I
> might contact.
> Thank you,
> Judy Adams
>
>
>
>
>
> _________________________________________________________
> DO YOU YAHOO!?
> Get your free @yahoo.com address at http://mail.yahoo.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 07:31:00 -0500
> From: smuzquiz@mt.gov
> Subject: Declere!
>
>
> From:    Muzquiz, Sherron
>
> Date:    September 28, 1998    4:04p
>
> Subject: Declere!
>
> Has anyone out there tried this product? It is supposed to
> combine deparaffinization,rehydration and unmasking. I
> really don't know and would like any information on this. If
> it works it would sure save me a lot of steps. Thanks in
> advance for any help on this. You can e-mail me directly if
> you would like to.
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 08:01:03 -0500
> From: Jamie Erickson <JErickson@genetics.com>
> Subject: Re[2]: (Fwd) RE: Permeablization.  Why the need?? -Reply -Re
> -Reply
>
> Simon,
>             I have not heard of anything like that,
> sorry for the lack of input.
>
> Jamie Erickson
> Genetics Institute
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 09:16:08 -0500
> From: Nancy Shellhorn <nancy.shellhorn@zymed.com>
> Subject: Background in Image analysis
>
> Has anyone experience background staining on Progesterone antibody which
> shows up on the CAS system but does not show up in IHC under the
> microscope? The sensitivity level of the image analyzer was set with a
> standard control. Any help with this would be greatly appreciated.
> Thank-you in advance for your help.
>
> Nancy Shellhorn
> Zymed Laboratories
> 458 Carlton Court
> South San Francisco, CA  94080
> 1-800-474-4494 ext. 9002
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 09:16:37 -0500
> From: Nancy Shellhorn <nancy.shellhorn@zymed.com>
> Subject: Beta Casein
>
> Has anyone heard of this antibody? Do you know what it is used for and
> where it may be purchased? Thanks again in advance for any help you may
> be able to offer.
>
> Nancy Shellhorn
> Zymed Laboratories, Inc.
> 458 Carlton Ct.
> South San Francisco, CA  94080
> 1-800-874-4494 ext.9002
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 09:17:03 -0500
> From: "D. Hammer" <hammerd@u.washington.edu>
> Subject: Re: special stain controls
>
> Kathy-Jean,
>
> Correction on my mail from last nite.
>
> The control slides are just dated (no accession #) They are filed in date
> order. The accessioned case slide has the date on it as well.
> They can be matched up by the dates.
>
> Hope that is clearer. :)
> Don
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Don Hammer, Administrative Director            UNIVERSITY OF WASHINGTON
> Hospital Pathology, Box 356100                     MEDICAL CENTER
> 1995 NE Pacific St.
> Seattle Washington, 98195                  ~Where Knowledge Comes To Life~
>
> (206) 548-6401 Fax: (206) 548-4928
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>
>
> > >
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 09:46:42 -0500
> From: "Patterson, Noelle" <PattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL>
> Subject: Black and White Microscopy Photos
>
> Histonetters,
>
> I need to shoot Black and White prints of Hematoxylin counterstained IHC
> developed with the DAB substrate/chromagen.  Normally, I take color slides
> and B&W prints are made from these.  So, I am asking for suggestions and
> pointers on how to take the best pictures.  What film speed and type do
> you
> recommend for clear pictures with good contrast?  What filters, if any,
> are
> needed?  What ISO/Reciprocity do people find work best?  Most likely, I
> will
> be shooting these pictures on an Olympus Vanox with 35 mm film.  These
> will
> need to be blown up to 5x7 prints to submit for publication.
>
> Thanks for all your advice.
>
> Sincerely,
> Noelle
> Noelle Patterson, M.S.
> NNMC/NMRI/ICBP
> Bethesda, MD 20889
> email:  pattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL
> FAX: 301-295-0376
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 10:30:24 -0500
> From: smuzquiz@mt.gov
> Subject: PVK!
>
>
> From:    Muzquiz, Sherron
>
> Date:    September 29, 1998    9:17a
>
> Subject: PVK!
>
> Becky I took your workshop in Salt Lake, really enjoyed it
> and I would love to get the Pierce Vander Kamp's. We do a
> lot of birds and you said this was really good for chlamydia
> and Rickettsia. I really would appreciate this. Thank you
> Sherron Muzquiz
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 10:39:04 -0500
> From: dave@biocare.net
> Subject: Factor VIII and Rat cross-reactivity
>
> Has anyone had experience with a rabbit anti-Von Willebrand Factor that
> stains rat tissue?  Does anyone know of any Factor VIII antibody that
> stains rat blood vessels?
>
> Thanks.
>
> dave@biocare.net
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 11:00:41 -0500
> From: "Suder, Joanne" <JC_Suder@fccc.edu>
> Subject: Retic problems
>
> Histoneters
>  I have been having some problems with  my Snooks reticulum stain. I
> purchase the kit from Poly Scinetific. The problems are precipitate and
> uneven staining of the reticulum fibers. Any suggestions would be
> appreciated.
>
> Thanks,
> Joanne
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 11:16:15 -0500
> From: "Hawkins, Hal" <hhawkins@SBI.UTMB.EDU>
> Subject: RE: Black and White Microscopy Photos
>
>
> I would suggest Plus-X film with a green filter.  If much more
> enlargement is needed, you can play with Technical Pan film.
>
> Hal Hawkins, UTMB Galveston
>
>  ----------
> From:  Patterson, Noelle
> Sent:  Tuesday, September 29, 1998 9:54 AM
> To:  Histonet
> Subject:  Black and White Microscopy Photos
>
> Histonetters,
>
> I need to shoot Black and White prints of Hematoxylin counterstained IHC
> developed with the DAB substrate/chromagen.  Normally, I take color
> slides
> and B&W prints are made from these.  So, I am asking for suggestions and
> pointers on how to take the best pictures.  What film speed and type do
> you
> recommend for clear pictures with good contrast?  What filters, if any,
> are
> needed?  What ISO/Reciprocity do people find work best?  Most likely, I
> will
> be shooting these pictures on an Olympus Vanox with 35 mm film.  These
> will
> need to be blown up to 5x7 prints to submit for publication.
>
> Thanks for all your advice.
>
> Sincerely,
> Noelle
> Noelle Patterson, M.S.
> NNMC/NMRI/ICBP
> Bethesda, MD 20889
> email:  pattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL
> FAX: 301-295-0376
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 11:45:48 -0500
> From: JanMinshew@aol.com
> Subject: Vendor list information
>
> Hello,
>
> This message is to my fellow vendors who monitor the Histonet.  I am
> trying to
> put together a list of contact names and email addresses for the companies
> that attend the NSH meetings.  There are lots of new developments that you
> will want to know about.   Please let me know who you are and how to reach
> you.  Thank you.
>
> Jan Minshew
> NSH Exhibitor Liaison
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 11:46:14 -0500
> From: Randy Tindall <rtindell@NMSU.Edu>
> Subject: Black and white microscopy photos
>
>
> Noelle,
>
> For a 5x7 blowup, I'd recommend a medium speed film such as Plus-X or TMax
> 100.  TMax will give the finer grain of the two, in case you will need
> larger pics later.  It will give you nearly grainless 11x14s under good
> conditions.  Tech Pan is usually overkill in situations like this, unless
> you need wall-size blowups, plus it's trickier to process evenly, requires
> a special developer, etc.
>
> For filters, the rule of thumb is that whatever color filter you choose
> will lighten that same color in the final print.  A red filter will make
> anything reddish relatively lighter than other colors, for example, and
> will darken anything that is greenish or bluish.
>
> Hope this is useful.
>
> Randy
>
> Randy Tindall
> Electron Microscope Laboratory
> Box 3EML--Biology
> New Mexico State University
> Las Cruces, NM  88003
>
> rtindell@nmsu (work)
> nrtindall@zianet.com (home)
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 11:46:43 -0500
> From: Heike Grabsch <h.grabsch@uni-koeln.de>
> Subject: Re: Black and White Microscopy Photos
>
> I do agree with Hal that you should use at least a green filter,
> sometimes it is even worth trying to use a red filter, but you definetly
> need a green one to get good contrast. then I would choose a technical
> pan film f.ex. from Agfa with ISO not higher than 100.
>
> Heike Grabsch, Germany
>
> Hawkins, Hal wrote:
> >
> > I would suggest Plus-X film with a green filter.  If much more
> > enlargement is needed, you can play with Technical Pan film.
> >
> > Hal Hawkins, UTMB Galveston
> >
> >  ----------
> > From:  Patterson, Noelle
> > Sent:  Tuesday, September 29, 1998 9:54 AM
> > To:  Histonet
> > Subject:  Black and White Microscopy Photos
> >
> > Histonetters,
> >
> > I need to shoot Black and White prints of Hematoxylin counterstained IHC
> > developed with the DAB substrate/chromagen.  Normally, I take color
> > slides
> > and B&W prints are made from these.  So, I am asking for suggestions and
> > pointers on how to take the best pictures.  What film speed and type do
> > you
> > recommend for clear pictures with good contrast?  What filters, if any,
> > are
> > needed?  What ISO/Reciprocity do people find work best?  Most likely, I
> > will
> > be shooting these pictures on an Olympus Vanox with 35 mm film.  These
> > will
> > need to be blown up to 5x7 prints to submit for publication.
> >
> > Thanks for all your advice.
> >
> > Sincerely,
> > Noelle
> > Noelle Patterson, M.S.
> > NNMC/NMRI/ICBP
> > Bethesda, MD 20889
> > email:  pattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL
> > FAX: 301-295-0376
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 12:16:20 -0500
> From: mcauliff@UMDNJ.EDU
> Subject: Re: Black and White Microscopy Photos
>
> Patterson, Noelle wrote:
> >
> > Histonetters,
> >
> > I need to shoot Black and White prints of Hematoxylin counterstained IHC
> developed with the DAB substrate/chromagen. Normally, I take color slides
> and
> B&W prints are made from these. So, I am asking for suggestions and
> pointers
> on how to take the best pictures. What film speed and type do you
> recommend
> for clear pictures with good contrast?  What filters, if any, are needed?
> What
> ISO/Reciprocity do people find work best?  Most likely, I will be shooting
> these pictures on an Olympus Vanox with 35 mm film. These will need to be
> blown up to 5x7 prints to submit for publication. Thanks for all your
> advice.
> >
> > Sincerely,
> > Noelle
> > Noelle Patterson, M.S.
> > NNMC/NMRI/ICBP
> > Bethesda, MD 20889
> > email:  pattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL
> > FAX: 301-295-0376
>
> Noelle:
>
> 	You will need a green filter, either a #11 (also known as an X1) or
> a
> #58. Without a green filter contrast will suffer quite a bit.
> 	For film try Kodak T-max 100 perhaps with some "extra" exposure, ASA
> 50
> or even 25. Manufacturers ASA settings are guidelines, not absolutes and
> correct exposure depends on the subject. Develope in T-max developer as
> per instructions for starters. If this does not give enough contrast try
> Kodak Tech Pan at ASA 25-50, developed in Kodak HC-110, dilution F, for
> 9 min at 24C. Good luck.
>
> Geoff
> - --
> ***************************************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane  Piscataway, NJ 08854
> voice: (732)-235-4583; fax -4029   e-mail: mcauliff@umdnj.edu
> ***************************************************************
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 12:17:06 -0500
> From: "Weems, Joyce" <JWEEMS@sjha.org>
> Subject: In search of Sandra
>
> Sandra Fite, I lost your Email address. Would you please get back with
> me?
> Thanks, Joyce :>)
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 13:15:24 -0500
> From: <carnek@dianon.com>
> Subject: Russ Allison
>
> Can anyone give me Russ Allison's e-mail address or Russ if you're out
> there
> please let me know.
>
> Thanks
> Kathy
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 14:30:54 -0500
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: IHC on bone
>
> John,
> 	Really can't help you we are experiencing the same problem with
> fixed and sucrose protected mouse brain. We have tried probe on slides
> poly
> l lysine are are going to try vectabond. we have better luck when we dry
> the
> slides overnight at RT. Will be anxious to hear from others!
> Cynthia
>
> > ----------
> > From: 	bakerj@umich.edu
> > Sent: 	Monday, September 28, 1998 7:13 AM
> > To: 	Histonet@pathology.swmed.edu
> > Subject: 	IHC on bone
> >
> > Dear Histonetters,  Recently messages regarding the drying of sections
> > before IHC have been routed via Histonet.  Does this also apply to
> > cryosections which will have IHC done to them?  I am cryosectioning bone
> > and get good sections but some of the tissue (bone) loosens from the
> slide
> > during the IHC.  I am using ProbeOn slides only.  My questions are:
> > Should
> > drying be done to the slides, and at what temperature and time period
> can
> > the slides be dried?    Should some other type of slide be used?
> Thanks,
> > John
> >
> > John A. Baker
> > The University of Michigan
> > Orthopaedic Research Laboratories
> > Histology Unit
> > 400 North Ingalls, G161
> > Ann Arbor, MI 48109-0486
> > my office 734-936-1635
> > lab office 734-763-9674
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 15:00:46 -0500
> From: Rebecca S Smith <bssvpisu@iastate.edu>
> Subject: RE: IHC on bone
>
> We have had troubles with the bone sections coming off in the past also.
> The best thing we have found is to use Silanized or Plus slides.  We drain
> the sections on the slides until the water has exited from under the
> section.  Sometimes this needs to be assisted with a tissue or a filter
> paper.  As soon as this has been accomplished we dry the sections onto the
> slides by placing them on a hot plate of 58 degrees, for at least 10 - 15
> minutes.  After this we check and see if there is any curling of the
> section.  If you see this now,  there is not much hope for that
> section....
> So... we cut another and try again.  The right balance between soaking the
> face of the block, floating on the water bath, draining, and drying flat
> seem to be the trick for us.  When we have a successful slide after the
> hot
> plate we leave the slide at room temp until we need to stain it.  At that
> time, we reheat the slides as we would any slide prior to
> deparaffinization
> and rehydration.  Good luck!!
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 15:10:24 -0500
> From: "christine.richardson" <christine.richardson@which.net>
> Subject: Re: subscribe digest
>
> subscribe
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 15:20:07 -0500
> From: "christine.richardson" <christine.richardson@which.net>
> Subject: Re: subscribe digest
>
> subscribe
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 15:34:14 -0500
> From: "christine.richardson" <christine.richardson@which.net>
> Subject: Re: digests
>
> digests
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 15:34:51 -0500
> From: "Patterson, Noelle" <PattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL>
> Subject: B&W photomicroscopy-Thanks
>
> Thanks to all for the timely and useful information, per my request.  I
> was
> able to get a hold of Kodak TMax100 and Kodak technical pan film ESTAR-AH
> Base black and white print film.  The Tech pan film expired in Oct. 1997,
> so
> I will only try that should I NEED it.  We have started shooting pictures
> with an ISO of 100 and reciprocity of 2 per the Olympus technical service
> recommendation.  I will let you know how it turns out.  I have the joy of
> training someone else while I am learning this.  Your help is greatly
> appreciated.
>
> Anyone wanting an email containing all the responses, please email me at
> the
> address below (or hit the reply).
>
> Respectfully,
> Noelle
> Noelle Patterson, M.S.
> NNMC/NMRI/ICBP
> Bethesda, MD 20889
> email:  pattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL
> FAX: 301-295-0376
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 15:59:21 -0500
> From: shelia Rogers <shelia@ortho.lsumc.edu>
> Subject: re: subscribe digest
>
>
> - ---------------------------------------------------------------
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 16:10:56 -0500
> From: "Kathy Oprea" <koprea@calc.vet.uga.edu>
> Subject: Wow, histonet is the best!
>
> Hello everyone,
> I wanted to post a public "Thank you" to Beverly Robinson with
> Richard Allen Scientific for the immersion oil samples. Apparantly
> she saw my request for help, here on the histonet, and was kind
> enough to voluntarily send me samples!!
> I have been able to gather both equipment and supply information,
> through inquiries posted by others. Histonet is such a great source
> of information for both immediate, and future use. Many thanks to all
> of you that take the time to be so informative.
>
> Kathy Oprea
> ************ Thank you ************
> * Kathy Oprea                     *
> * University of Georgia           *
> * College of Veterinary Medicine  *
> * Athens, GA 30602-7388           *
> * KOPREA@calc.vet.uga.edu         *
> The worker who's never said "Thank
> God it's Friday," probably never
> really worked.
> ************************************
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 16:20:51 -0500
> From: Jamie Erickson <JErickson@genetics.com>
> Subject: RE: IHC on bone -Reply
>
> John,
>           I've done some bone(mouse paws) IHC
> on our Ventana Techmate. I found that even on
> charged slides the paws fell off. I tried a slide
> called superfrost plus gold slides from Erie
> Scientific. Cryosections where sectioned put on
> slides (Gold) and let air dry for 2-5 minutes then
> fixed in Cold -20 C acetone for 1 minute. I
> stored them @ -20 till stained and the sections
> didn't detach from the slide.
>      The slide drying for paraffin sections (60
> degrees C for an Hour) I would not recommend
> for your cryosections.   FYI.
>
> Jamie Erickson
> Genetics Institute
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 17:00:56 -0500
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: Declere!
>
> Sherron,
> 	I have no experience with this product, but can tell you that I have
> forgotten to defparaffinize before HIER and the procedure I was using at
> the
> time still worked. I would not recommend it for routine use because I
> haven't compared it with the usual methodology.
> Cynthia
>
> > ----------
> > From: 	smuzquiz@mt.gov
> > Sent: 	Monday, September 28, 1998 5:04 PM
> > To: 	histonet@pathology.swmed.edu
> > Subject: 	Declere!
> >
> >
> > From:    Muzquiz, Sherron
> >
> > Date:    September 28, 1998    4:04p
> >
> > Subject: Declere!
> >
> > Has anyone out there tried this product? It is supposed to
> > combine deparaffinization,rehydration and unmasking. I
> > really don't know and would like any information on this. If
> > it works it would sure save me a lot of steps. Thanks in
> > advance for any help on this. You can e-mail me directly if
> > you would like to.
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 17:11:43 -0500
> From: Rebecca S Smith <bssvpisu@iastate.edu>
> Subject: RE: IHC on bone -Reply
>
> Sorry, I didn't notice we were talking about frozens.
>
>
> At 05:17 PM 9/29/98 -0400, Jamie Erickson wrote:
> >John,
> >          I've done some bone(mouse paws) IHC
> >on our Ventana Techmate. I found that even on
> >charged slides the paws fell off. I tried a slide
> >called superfrost plus gold slides from Erie
> >Scientific. Cryosections where sectioned put on
> >slides (Gold) and let air dry for 2-5 minutes then
> >fixed in Cold -20 C acetone for 1 minute. I
> >stored them @ -20 till stained and the sections
> >didn't detach from the slide.
> >     The slide drying for paraffin sections (60
> >degrees C for an Hour) I would not recommend
> >for your cryosections.   FYI.
> >
> >Jamie Erickson
> >Genetics Institute
> >
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 17:47:19 -0500
> From: conmac@cc.usu.edu (connie mcmanus)
> Subject: Re: Beta Casein
>
> Nancy,
> My husband works with milk proteins (he does EM on them) and I believe he
> had a B Casein antibody. It was difficult to come by.  If you e-mail me
> privately I will give you his e-mail so you can ask him yourself.
>
> Connie McManus
> Veterinary Diagnostics Lab
> Utah State University
> Logan, Utah
>
>
> >Has anyone heard of this antibody? Do you know what it is used for and
> >where it may be purchased? Thanks again in advance for any help you may
> >be able to offer.
> >
> >Nancy Shellhorn
> >Zymed Laboratories, Inc.
> >458 Carlton Ct.
> >South San Francisco, CA  94080
> >1-800-874-4494 ext.9002
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 20:09:36 -0500
> From: conmac@cc.usu.edu (connie mcmanus)
> Subject: MMA shortages and suppliers
>
> My boss asked me to set up undecalcified bone ... a first for this lab...
> and being familiar with MMA, that's what I'm doing.   I ordered some last
> July &  I STILL haven't received any of this stuff.  I've been wondering
> why... know I know ... a shortage.  sheesh.   hopefully we will receive it
> soon.
>
> Years ago I ordered my MMA directly from Rohm & Haas, but i heard they
> don't
> sell in quantities less than truck loads anymore.  Where do you MMA users
> recommend getting this stuff?  Thanx in advance
>
> Connie McManus
> Veterinary Diagnostics Lab
> Utah State University
> Logan, UT
>
>
>
> >     FYI: I received a case of MMA from Fisher today that I ordered in
> >     April!Maybe the shortage is over.
> >
> >     Also, instead of disposing the last solution of infiltrating medium
> I
> >     tried making prepolymerized molds with it.They polymerized faster
> than
> >     the freshly made solution and came out fine!
> >
> >     Andrea Kelly
> >     Albany Medical College
> >
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 20:28:08 -0500
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: Factor VIII to rat
>
> We used Dako's antibody (a rabbit antihuman) on mouse tissue with
> excellent results, probably would work with rat. WE did not buy
> prediluted,
> just the purified.   We also did Carnoys sans chloroform, and no antigen
> retrieval.  A delight!
>
> Gayle Callis
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 20:29:36 -0500
> From: "Nancy Klemme" <nancy.klemme@sakuraus.com>
> Subject: Formalin Neutralization
>
> I am posting this communique for a friend who is not on the Histonet.
>
> Dear Histonet Subscriber,
>
> This note is in response to a recent inquiry concerning the
> neutralization of formalin. While it is true that there are several
> companies that offer products that claim to neutralize completely, very
> few can offer any substantiation with respect to this claim.
>
> Before purchasing any treatment technology, check the following:
> o Is the product certified by the California EPA Department of Toxic
> Substances Control? Regardless of what geographic area your institution
> is located, this certification is important because it guarantees that
> the technology has been thoroughly tested by an unbiased government
> institution and found to perform in accordance to the manufacturers
> claims. It also assures that no other toxic substances are generated
> while in process of neutralizing the formalin.
> o Once neutralized, does the treated formalin have an acceptable pH for
> disposal into the drain or does it require an adjustment first. In
> accordance to 40CFR 460.12, the pH must be tested prior to disposal. If
> any pH adjustments have to be made, they can be timely as well as
> costly.
> o The neutralized solution should also be tested for any residual
> formalin. The test can be as simple as a dip stick method that matches a
> color scale for the final determination. The desired result is to get as
> close to 0 ppm as possible.
> o If the treatment technology generates any solid waste in the form of
> precipitate or sludge, it must be separated from the liquid prior to
> disposal. Solid waste is not acceptable for disposal to the drain.
>
> The utilization of uncertified treatment technologies is always risky
> because there is no assurance that the product is actually doing what it
> was sold to do. Testing for pH and residual formalin is a great way to
> verify its effectiveness in neutralizing aldehyde waste. It also
> satisfies any potential regulations that may have been put into effect
> and enforced by local wastewater treatment plants.
>
> If any of you have any additional questions, please don't hesitate to
> send me an E-mail. My address is tedbryan@earthlink.net.
>
> Regards,
> Ted Bryan
>
>
>
> Nancy Klemme, HT(ASCP)
> Customer/Product Support Mgr.
> Sakura Finetek USA, Inc.
> 1750  West 214th Street
> Torrance, CA  90501
>
> Web Page = www.sakuraus.com
> e-mail = nancy.klemme@sakuraus.com
> Phone = 800/725-8723
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 20:56:45 -0500
> From: Inka Tertinegg <inka@idirect.com>
> Subject: JB-4 problems
>
> I'm relatively new to plastic sectioning and am having a difficult time
> using JB-4 plus. Most recently, I have blocks the consistency of gummi
> bears. I thought that this was due to the degradation of
> catalyst(benzoyl peroxide) since the product sheet mentions that the
> catalyst will deteriorate with time, but having done a quick test using
> varying amounts of catalyst and accelerator and getting soft blocks
> still, I'm thinking its something that's happened to the monomer, but
> what? Can anybody out there help me?
> Thanks in advance,
>
> Inka Tertinegg
> Dept. of Ophthalmology
> University of Toronto
> inka@idirect.com
>
>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 21:45:18 -0500
> From: pfutrell@juno.com (PAMELA S FUTRELL)
> Subject: IHC Antibodies
>
> Hello,
>     I was wondering if anyone out there in Histo land has worked with the
> CD-41 , CD-61 and
> Cycline D1  antibodies in paraffin sections.  I am having problems
> getting them to work.  What kind of
> controls are you using and what kind if antigen retrieval are you doing?
> Any information would be
> helpful.   Thanks
>
> Pam Futrell
> Lakeland Regional Medical Center
> Lakeland, FL
> (941) 687-1007
> pfutrell@gte.net
> ___________________________________________________________________
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>
> ----------------------------------------------------------------------
>
> Date: 29 Sep 1998 23:45:25 -0500
> From: "LEROY BROWN" <lhbhcs@pioneernet.net>
> Subject: miles cryostat
>
> Does anyone know who would have a manual for a Miles, Tissue-tek cryostat?
> It's an older unit.
> thanks
> Roy
>
> HCS
> 85 SE 8th Ave.
> oak harbor, WA 98277
> 1-800-732-8158
> 1-360-675-5820 fax
>
>
>
> Here are the messages received yesterday!