Negative controls for immunos

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From:"Barnhart, Tammy" <> (by way of histonet)
To:histonet <>
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                Question, a new CAP standard (08.2257) states, " Are
negative controls used for each antibody?".  We were wondering how this
standard is applied at other labs.  At this time we only run negative
controls on different tissue blocks used, both patient specimens and
controls.  We use several multitissue control blocks ( one for CDs, one for
keratins, etc) and will run different negative controls for different serum
types (ie: rabbit or mouse).  If we interprete this standard literally, we
will greatly increase the number of slides handled and, almost, double our
reagent costs.  Which brings up a second question, how do you handle your
negative controls?  Do you run them completely, using serum instead of
primary antibody, or do an abbreviated procedure?  All reponses would be
appreciated.  Thanks in advance,

Tammy Barnhart

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