IHC, ICAM -1 in Rat tissue

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From:"Harclerode, Donna" <DHarclerode@ligand.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Hi Tim,

Pharmingen's ICAM-1(CD54) (cat 22491D) works  well in rat paraffin
embedded tissue.  I use 2.5µg/ml of primary diluted in  Dako® antibody
diluent overnight at 4oC with citrate microwave pretreatment.  I use
biotinolated polyclonal anti mouse secondary (adsorbed to rat) and LSAB
(Dako®) with DAB.  (The catalog has the protocol in the back)  Adjust
for your particular  needs.  They have many antibodies made specifically
for use on rat and mouse tissues.

Donna Harclerode HT, ASCP, HTL, QIHC
Associate Scientist
Ligand Pharmaceuticals
San Diego, CA

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