Dear Histonetters,
     I have been following the ongoing discussion about saponin vs triton
or tween.  I have recently switched to saponin instead of Triton 100-X for
some of my protocols.  I have noticed a difference in the amount of
background.  I didn't understand all the reasons why until I read some of
your responses.  I use .1% in PBS for my blocking and primary and secondary
antibodies. I use Vector ABC elite kits. I use only PBS with my complex.  I
would like to know if there is way to know what % of detergents to use or
is it trial and error. I used to use .2% or .3% Triton 100-X. My samples
are frozen sections of decalcified dog teeth and paraffin embedded rat
mandibles.
                                            Thanks,
                                             Pat Landry, U of Michigan/Dental