[Fwd: Anyone out there using Omnifix 2000 for tissue fixation?]

<< Previous Message | Next Message >>
From:Diana Goodwin <slipper@net-gate.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
Content-Type:text/plain; charset="us-ascii"

Diana Goodwin, HT
Trenton, NJ

From: obadiam@moffitt.usf.edu (M. Obadia)
Newsgroups: sci.med.pathology
Subject: Anyone out there using Omnifix 2000 for tissue fixation?
Date: Thu, 01 Oct 1998 06:55:45 -0500
Organization: University of South Florida
Message-ID: <obadiam-0110980655450001@pc-mcc-3041-2.moffitt.usf.edu>
NNTP-Posting-Host: pc-mcc-3041-2.moffitt.usf.edu
Xref: newsfeed.slurp.net sci.med.pathology:8732
MIME-Version: 1.0

If any of you are using Omnifix 2000 tissue fixative...can you please tell
me what protocol you are using for tissue dehydration, clearing and
paraffin embedding? Specifically, the length of times in each (ETOH and
toluene/xylene) as well as length of time and temperature in paraffin.

I am processing brain tumor tissue (rat and human) and do basic histology
and immunohistochemistry. I'm just looking for the optimal processing. Any
advice is much appreciated!

Thank you!

<< Previous Message | Next Message >>