We've been using a polyclonal antibody to the A-subunit of human
coagulation Factor XIII (Factor XIIIa).  It seems to work fine without
any pretreatment.  We used skin, placenta and a capillary hemangioma for
controls.  We tried digestion and it didn't seem to make much
difference.  However, the literature shows many procedures using either
trypsin or antigen retrieval methods.  Could someone who has work a lot
with this antibody please enlighten me?

Thanks