CD30

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From:Dave Tacha <dtacha@ncal.verio.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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CD30 Tech Tips

Probably the optimum control would be a two-in one;  Hodgkin's and
anaplastic large cell lymphoma (APLC).  As far pretreatment, I've tried
digestion and heat retrieval methods at 6.0 pH and 9.0 pH.  I found that
using a citrate buffer at pH 6.0 works the best in our hands.  I
personally use a cocktail of CD30 antibodies. I get superior staining
versus single-clone antibodies such as BerH2.  I also incubate both CD15
and CD30 at one hour.  I don’t think shortcuts should be taken,
considering the consequence to the patient, if the incorrect diagnosis
is given.  Below is a panel used for Hodgkin’s vs APLC.


                        CD15    CD30       BLA36       ALK1

Hodgkin’s           +           +               +                -
APLC                 -            +              +                -

A lot of progress has been made for the treatment of Hodgkin’s.  The
prognosis is much better than anaplastic large cell lymphoma, which
requires a much more aggressive treatment.

CD30 and CD15 can be technically problematic.  Optimum protocols are
essential!

I believe CD stands for "Cluster Designation".  A true cluster
designation is usually assigned at the International Workshops (IW).
Once the antibody has gone through a gauntlet of testing, they assign
“CD” numbers (stamp of approval).  I believe there are about 166 numbers
that have been assigned.


David Tacha




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