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From:"Hagerty, Marjorie A." <> (by way of histonet)
To:histonet <>
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<<Found out that if I ran the B5 fixed tissues through Lugol's and
before immunostaining the CD30 staining was blocked or the antigenic sites
were modified,  causing false negatives.  Has anyone else observed this
Mark - Thank you for the very helpful CD30 info. Yes, I have found the same
thing with B5 fixed tissue for immuno. I discovered it while doing ER/PR
staining. The culprit seems to be the sodium thiosulfate. We use a
xylene/iodine solution to de-B5 before immunostaining. 95% alcohol in the
run-down removes the iodine crystals. We used to de-B5 after staining but
this is easier to remember.
Thanks again.

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