Re: [Histonet] stain

From:"Bryan Llewellyn"

Altmann's acid fuchsin picric acid is a histological stain for mitochondria.

It requres the tissue to be fixed in a non-acid dichromate fixative, then 
post-chromed for some time.  The tissue is then processed by paraffin and 

Staining is in aniline water saturated with acid fuchsin, which is applied 
much like carbol fuchsin in a ZN, i.e. heated.  Diffeerentiation is with 
picric acid.

The method requires considerable skill from the technologist, but produces 
red mitochondria on a yellow background.

I suggest that if you just want to demonstrate mitochondria, do a succinate 
dehydrogenase instead, it is much easier and much faster.

If you want, I can look up the details and send them on, but I recall they 
used to be in Culling's book.

Bryan Llewellyn

----- Original Message ----- 
From: "Patsy Ruegg" 
Sent: Wednesday, November 05, 2008 2:27 PM
Subject: [Histonet] stain

> Anyone familiar with this stain?
> Altmann acid-fushsin picric. This stain is a histochemical stain for
> mitochondria in intestine.
> Best regards,
> Patsy
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email
> web site
> This email is confidential and intended solely for the use of the 
> Person(s)
> ('the intended recipient') to whom it was addressed. Any views or opinions
> presented are solely those of the author. It may contain information that 
> is
> privileged & confidential within the meaning of applicable law. 
> Accordingly
> any dissemination, distribution, copying, or other use of this message, or
> any of its contents, by any person other than the intended recipient may
> constitute a breach of civil or criminal law and is strictly prohibited. 
> If
> you are NOT the intended recipient please contact the sender and dispose 
> of
> this e-mail as soon as possible.
> _______________________________________________
> Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>