Altmann's acid fuchsin picric acid is a histological stain for mitochondria.
It requres the tissue to be fixed in a non-acid dichromate fixative, then
post-chromed for some time. The tissue is then processed by paraffin and
Staining is in aniline water saturated with acid fuchsin, which is applied
much like carbol fuchsin in a ZN, i.e. heated. Diffeerentiation is with
The method requires considerable skill from the technologist, but produces
red mitochondria on a yellow background.
I suggest that if you just want to demonstrate mitochondria, do a succinate
dehydrogenase instead, it is much easier and much faster.
If you want, I can look up the details and send them on, but I recall they
used to be in Culling's book.
----- Original Message -----
From: "Patsy Ruegg"
Sent: Wednesday, November 05, 2008 2:27 PM
Subject: [Histonet] stain
> Anyone familiar with this stain?
> Altmann acid-fushsin picric. This stain is a histochemical stain for
> mitochondria in intestine.
> Best regards,
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
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> web site www.ihctech.net
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