I had a little problem once with Schiffs.I was making up a new batch (I believe about 2 liters) and had just finished boiling the water. I was in a hurry (often the culprit in disasterous lab events) and forgat that impure solvents have a lower boiuling point. When I added the basic fuchsin, it erupted like a volcano!Now, to get the full picture, you must envision a newly remodeled lab. New tile floor. Newly painted cupboards. Fresh paint on the wall. And, of course, the lab director a planning walk-through show-off tour of the new facility.Bucket and mop to try to remove most of the excess. Gallons of acid alcohol. And, yes, 10% bleach solution for the fresh paint and the cabinets. It took all day, but I got it looking good as new.The next week, someone broke a container of eosin on the floor. My boss forever blamed me for the orange-pink arising every time they mopped the floor...________________________________From: "renafail@bellsouth.net" To: "Swain, Frances L" ; Emily Sours ; histonet@lists.utsouthwestern.eduSent: Friday, November 7, 2008 6:05:52 PMSubject: RE: [Histonet] schiff'sA Liter!!! It took most of a day to clean 40 ml dropped in a dishwasher to which the tech added extra multiterge, which caused the dishwasher to overflow. A colorful event. My sympathyRena Fail-------------- Original message from "Swain, Frances L" : --------------=0A> If you have a staining problem from the spill a weak solution of bleach> usually clears up any Schiff's that is dropped on the bench, floor, hands,> etc.> > Frances L. Swain HT(ASCP) A. A. S.> Special Procedures Technician> Department of Orthopaedic Surgery> Center for Orthopaedic Research> Barton Research Building 2R28> 4301 West Markham Street> Little Rock AR 72205> (501) 686-8739 PHONE> (501) 686-8987 FAX> swainfrancesl@uams.edu email> -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours> Sent: Friday, November 07, 2008 1:12 PM> To: histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] schiff's> > Schiff's reagent is my nemesis. It's clear until it gets on anything> outside of the bottle.> Including the floor, clothing, and our fume hood.> (Our post-doc had a little accident. Actually a rather large one, involving> 1 L of Schiff's.)> > Emily> > On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger wrote:> > > We are using Shiffs reagent. When you put a drop of formalin in Shiffs> > reagent it turns purple.> >> > Roger Charles> > Microbiologist> > Pennsylvania Veterinary Laboratory> > 2305 N Cameron St=0A> > Harrisburg, PA 17110> > 717-787-8808> >> > -----Original Message-----> > From: histonet-bounces@lists.utsouthwestern.edu [mailto:=0A> > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda=0A> > Sent: Friday, November 07, 2008 1:31 PM> > To: histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Is it Formalin-Without Smelling It?> >> > Happy Friday Everyone!!!!!> > Silly question: Is there an easy way to tell if a solution in a container> > is/is not formalin without smelling it?????> > Thanks,> > Wanda> >> > WANDA G. SMITH, HTL(ASCP)HT> > Pathology Supervisor> > TRIDENT MEDICAL CENTER> > 9330 Medical Plaza Drive> > Charleston, SC 29406> > 843-847-4586> > 843-847-4296 fax> >> >> > _______________________________________________> > Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> >> > _______________________________________________> > Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> >> > > > -- > "You would know her for all the things she was...a woman who knew her way in=0A> and out of every new book without being singed, pinched, bumped or tickled> by any line or chapter."> John O'Hara, Appointment in Samarra=0A> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > Confidentiality Notice: This e-mail message, including any attachments, is for > the sole use of the intended recipient(s) and may contain confidential and > privileged information.=A0 Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|