Check the original source of your method. If it's a typed sheet
with= no published source (such as a book or a peer-reviewed paper),
forget= it and start again.
Malachite green
is used = mainly in bacteriology. Recent and traditional
applications are summar= ized, with references in Conn's
Biological Stains.= For general-purpose counterstaining to
follow PAS, I recommend any ha= emalum nuclear stain (eg
Mayers's, Ehrlich's, Gill's) followed= by fast
green FCF (0.1-0.2% in 1-2% acetic acid). T= his gives you
blue nuclei and green cytoplasm and collagen. The green = has a
bluish cast, but both colours contrast well with PAS. For a wi= de
variety of anionic counterstains in various colours, see Presnell J K & Schreibman MP (1997) Humason's Animal Tissue
Techniques,= 5th ed. Baltimore: Johns Hopkins Univ Press.
Earlier editions of = Humason's have the same
information.
John K iernan
Anatomy, UWO
London, Canada
- -
-
= ----- Original Message -----
From:
"napoli@siscom.net" <napoli@siscom.net>
Date: Sunday,
November = 23, 2008 10:37
Subject: [Histonet]
malachite green coun= terstain
To:
histonet@lists.utsouthwestern.edu
<= BR>>
Anyone have knowledge of using malachite green as a
= >
counterstain for PAS stains for fungus? I have been getting< BR>> poor results using it as it tends to wash out very
quickly=
> in the dehydrating process before
coverslipping. Any = ideas?
> It seems as though it
hasn't worked as well l= ately as it has
> in the
past...getting mixed succes= ses. Something is
wrong
> with my voodoo!
>
> Puzzled dermatotech
>
>
<= BR>>
>
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