I"ve seen underfixed tissue stain with an ugly background everywhere for
kappa/lambda (so much that you can't tell what's real staining and what
isn't).  I think it depends on your retrieval.  Especially if you're
digesting for retrieval, and it wasn't properly fixed, I can imagine all
kinds of staining issues.

On Wed, Nov 12, 2008 at 10:00 AM, Gudrun Lang  wrote:

> Thank you for your answers. An additional question: Can someone confirm,
> that underfixed tissue shows false positive kappa and lambdastaining?
>
> Bye
> Gudrun
>
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun
> Lang
> Gesendet: Mittwoch, 12. November 2008 16:45
> An: histonet@lists.utsouthwestern.edu
> Betreff: [Histonet] experiences with kappa/lambda
>
> Hi listmembers,
>
> Please tell me about your experiences with the IHC-demonstration of kappa
> and lambda on FFPE-sections.
>
> We have some troubles about inconsistend staining and therefore false
> results about the monoclonality of tumors.
>
> Bye
>
> Gudrun
>
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