Hi Gudrun. We have a fair bit of success with kappa/lambda IHC. We use
polyclonal antibodies, a heat citrate pretreatment. The caveat is that we
have several different titers in use - we have a titer for lymph nodes, one
for bone marrows/decaled tissue, one for tissue fixed in zinc formalin,
etc.... We have pretty good correlation with our flow lab. I believe that a
couple of years ago Rich Cartun published a paper on his methods & he has
good correlation, too. It does require a pathologist with good knowledge of
heme path & patience to work out the optimum staining with the lab.


Patti Loykasek BS, HTL, QIHC
Clinical IHC Supervisor
PhenoPath Laboratories
Seattle, WA




> Hi listmembers,
> 
> Please tell me about your experiences with the IHC-demonstration of kappa
> and lambda on FFPE-sections.
> 
> We have some troubles about inconsistend staining and therefore false
> results about the monoclonality of tumors.
> 
> Bye
> 
> Gudrun
> 
> _______________________=5F_______________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet