Re: [Histonet] experiences with kappa/lambda

From:Patti Loykasek

Hi Gudrun. We have a fair bit of success with kappa/lambda IHC. We use
polyclonal antibodies, a heat citrate pretreatment. The caveat is that we
have several different titers in use - we have a titer for lymph nodes, one
for bone marrows/decaled tissue, one for tissue fixed in zinc formalin,
etc.... We have pretty good correlation with our flow lab. I believe that a
couple of years ago Rich Cartun published a paper on his methods & he has
good correlation, too. It does require a pathologist with good knowledge of
heme path & patience to work out the optimum staining with the lab.

Patti Loykasek BS, HTL, QIHC
Clinical IHC Supervisor
PhenoPath Laboratories
Seattle, WA

> Hi listmembers,
> Please tell me about your experiences with the IHC-demonstration of kappa
> and lambda on FFPE-sections.
> We have some troubles about inconsistend staining and therefore false
> results about the monoclonality of tumors.
> Bye
> Gudrun
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