Incubate with one antibody and use DAB as the chromagen, then go back and incubate with the second antibody and use a colored chromagen such as AEC. =0APaula----- Original Message ----From: "Edwards, R.E.." To: histonet Sent: Monday, November 3, 2008 8:08:46 AMSubject: [Histonet] double troubleOK chaps, which in your opinion is the  best double labelling method when one  is  using primary antibodies raised in the  same  species, I am aware that Vector labs supply such a kit; many  thanks..                                  =A0   Cheers                            =A0               Richard Edwards            =A0                                           University of Leicester                =A0                                                           U.K....          =A0                   _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet