CitriSolve works somewhat better than other d-Limonene xylene substitutes because it is a hybrid.
It contains d-Limonene at 70% (or 50% according with other recipes) + quaternary amnine mixture (or aliphatic hydrocarbons in another recipe) at 25% (or greater %) and 2-butoxyethanol at 5%. This 2-butoxyethanol is a suspected carcinogen with a TWA of only 25 ppm.
CitriSolve is described as corrosive to the eyes. It costs 2.31 times more than Xylene and
I personally would not use it.
--- On Wed, 10/8/08, kbowden wrote:
Subject: Re: [Histonet] citrisolve?
To: "Patten, Nicole (NIH/NIAAA) [F]"
Date: Wednesday, October 8, 2008, 5:37 PM
I have used Citrisolve for serveral years. I don't think anything
compares to Xylene, but I do think this comes very close. I change it
about once a week. It pretty much deparaffinizes in the same amount of
time. Disposal is dependent on the regulations in your area. When I
started using it our Environmental Health and Safety said pour it down
the drain, but as time goes by and with more regulations now I have to
collect it and have it removed with hazardous waste.
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
9500 Gilman Dr. 0630
La Jolla, CA 92093-0630
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Patten, Nicole (NIH/NIAAA) [F] wrote:
> Does anyone have any experience using Citrisolve as a Xylene alternative
> to deparaffinize tissue sections? Do I use it just like Xylene for the
> same amount of time? Can it be reused or should I use new Citrisolve
> each time? How do I dispose of it?
> Any help at all would be much appreciated. Thanks!
> Nicole J. Patten
> Post-Baccalaureate Fellow/IRTA
> National Institutes of Health
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