Alkaline  phosphatases  are  enzymes  that  catalyse the hydrolysis of
   phosph=  ate  esters.  That  includes  phosphate  esters of tyrosine
   (formed  by  acti= on of tyrosine kinases). This hydrolysis restores
   the phenolic side-ch= ain of tyrosine. The traditional histochemical
   blocking reactions for = tyrosine
   are:
      Acylation,   with      acetic anhydride of benzoyl
   chloride
   =   Iodination,   with
   iodine
      Nitrati=   on,   with
   tetranitromethane.
None of these reactions are speci= fic for
   tyrosine,  but  they  will  prevent  histochemical reactions that g   enerate  coloured  products with that amino acid, such as the Millon
   tes=    t.
 
John   Kiernan
Anatomy,
   UWO
= London, Canada
= =
   =

---Original       Message--=       -
Soldana
   Cristiana ICH               <cristiana.soldani@hu   manitas.it>
Date:  Thursday,  November  13, 2008
   11:=        02
Subject:       [Histonet]       alkaline
   phosphatase
To: hi   stonet@lists.utsouthwestern.edu

>  I would
   li=   ke   to   know   if   someone   used   alkaline  phosphatase  on
   
>    cryo=    statictissue   section   to   block   the
   tyrosine.
>   
&=   gt;   I  have  alkaline
   phosphatase    from    shrimp    (sigma)    but    I   don't   know   how
> many units I have to use, the temperature, the
   =      time....
>      
>     Many
   thanks
&= gt; 
>
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