Thank you Terry .. and thanks to everyone who replied to me about this ...
On Thu, Oct 2, 2008 at 10:37 AM, Marshall Terry Dr, Consultant
> 1.5% sounds homeopathic to me.
> There will be more depression of freezing point due to the salts in
> buffered formalin than anything.
> In the excerpt you gave Sate, antifreeze worked best (surprise
> It's hard to imagine with modern transport that things are going to hang
> around for long enough and at such a low temperature that crystals are
> going to form.
> PS I had a picture of the freezing artefact once but have lost it. If
> someone has one I sure would appreciate it.
> -----Original Message-----
> From: email@example.com
> [mailto:firstname.lastname@example.org] On Behalf Of Sate
> Sent: 02 October 2008 15:58
> To: email@example.com
> Subject: [Histonet] Winterize formalin?
> Dear Histonetters,
> A colleague from Boston posted this inquiry to our dermatopathology
> list: "Does anyone [have] the formula to winterize formalin? We were
> 15 ml of alcohol to 1000 ml of 10% buffered formalin. It used to work
> but is not working now at -20" ..
> Interestingly, I found out that we do not "winterize" formalin up here
> in Winnipeg, Canada (also jokingly referred to as "Winterpeg"). I am
> wondering if anyone on the list "winterizes" formalin .. I found the
> following abstract of a Japanese paper by searching in Pubmed
> Thank you ..
Sate Hamza, MD, FRCPC
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