When I was in Prince George, BC we used to tell our satellite labs to dilute
the NBF 20% with ethanol . This worked fine for us and below -20C. At
really cold temperatures it turned slushy rather than solid, but we rarely
got ice crystal holes.
I used to live and work in Winnipeg. I was the section head in histology at
the HSC from 67-74. How are things there, now?
----- Original Message -----
From: "Sate Hamza"
Sent: Thursday, October 02, 2008 7:58 AM
Subject: [Histonet] Winterize formalin?
> Dear Histonetters,
> A colleague from Boston posted this inquiry to our dermatopathology
> list: "Does anyone [have] the formula to winterize formalin? We were
> 15 ml of alcohol to 1000 ml of 10% buffered formalin. It used to work but
> not working now at -20" ..
> Interestingly, I found out that we do not "winterize" formalin up here in
> Winnipeg, Canada (also jokingly referred to as "Winterpeg"). I am
> if anyone on the list "winterizes" formalin .. I found the following
> abstract of a Japanese paper by searching in Pubmed
> Thank you ..
> Sate Hamza, MD, FRCPC
> Winnipeg, Canada
> Histonet mailing list
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