R. Tellyesniczky published more than one
fixative, 1898-190= 5. His best-known one translates into
AFA or FAA: mostly ethyl alcoh= ol, with about 4% formaldehyde
and about 5% acetic acid. It was = reinvented in 1937 by David
Bodian (Anat. Rec.
69: 153-162) as the best fixative for
subsequent = staining of axons with his 1936 protargol method
(Anat. Rec<= /EM>. 65:
89-97). AFA mixtures are gene= rally better than aqueous
formaldehyde fixatives if you need to stain no= rmal or regenerating
axons in paraffin sections with any silver method.
Bob Richmond may remember Bodian from Johns
H= opkins. Bodian made important contributions to knowledge of
functional= human neuroanatomy from clinico-pathological correlations
in people wit= h poliomyelitis. He correlated the positions of
virus-killed = motor neurons in the spinal cord with the
muscles that were paralysed b= efore death. This was a big job! The
adult human spinal cord is 45 cm = long, and neuronal
columns can be appreciated only in transv= erse sections.
Neuroanat= omist at
= = =
Messag= e -----
From: pam johnson
<firstname.lastname@example.org><= BR>Date: Tuesday,
November 18, 2008 12:14
Subject: [= Histonet]
To: email@example.com .edu
> Does anyone have a
recip= e for Telly's fixative?
Thanks,<= BR>> Pam
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