R. Tellyesniczky published more than one
   fixative, 1898-190= 5. His best-known one translates into
   AFA  or  FAA: mostly ethyl alcoh= ol, with about 4% formaldehyde
   and  about  5%  acetic  acid. It was = reinvented in 1937 by David
   Bodian          (Anat.         Rec.         69:   153-162)   as   the   best  fixative  for
   subsequent  =  staining  of  axons  with  his  1936  protargol  method
   (Anat.   Rec<=   /EM>.  65:
   89-97).   AFA   mixtures   are   gene=  rally  better  than  aqueous
   formaldehyde  fixatives  if you need to stain no= rmal or regenerating
   axons    in    paraffin    sections   with   any   silver   method.   
 
Bob  Richmond may remember Bodian from Johns
   H=  opkins.  Bodian  made  important  contributions  to knowledge of
   functional=  human neuroanatomy from clinico-pathological correlations
   in  people  wit=  h  poliomyelitis.  He  correlated the positions of
   virus-killed =  motor  neurons  in  the  spinal cord with the
   muscles  that were paralysed b= efore death. This was a big job! The
   adult  human  spinal  cord  is  45 cm = long, and  neuronal
   columns   can   be   appreciated  only  in  transv=  erse  sections.
   
 
John   Kiernan
Neuroanat=   omist  at
   UWO
London,    Canada
     http://instruct   .uwo.ca/anatomy/530/
=   =   =
-----  Original
   Messag= e -----
From: pam johnson
   <jhnspam@aol.com><=     BR>Date:    Tuesday,
   November   18,   2008   12:14
Subject:  [=  Histonet]
   Telly's      fixative
To:     histonet@pathology.swmed   .edu

>  
>  Does  anyone  have a
   recip=  e  for  Telly's fixative?
> 
>
   Thanks,<=             BR>>            Pam
>
   _____________   _______________________   5F=  __________
>  Histonet  mailing
   list>
   Histonet@lists.utsouthwestern.edu
>           htt   p://lists.utsouthwestern.edu/mailman/listinfo/histonet

   CB= R>