Problem solved. jane
"Life is short - make haste to be kind"
Dr. Jane Flinn
Director, Undergraduate Neuroscience Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA 22030
Phone: 703-993-4107
Fax: 703-993-1359
----- Original Message -----
From: Jane M Flinn
Date: Tuesday, October 14, 2008 1:33 pm
Subject: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa
> We are running a congo red stain and have sodium chloride crystals
> precipataing onto the slide. Does anyone know why and how we can
> fix this? jane
>
> "Life is short - make haste to be kind"
>
> Dr. Jane Flinn
> Director, Undergraduate Neuroscience Program
> George Mason University, 3F5
> 4400 University Dr.
> Fairfax, VA 22030
> Phone: 703-993-4107
> Fax: 703-993-1359
>
>
> ----- Original Message -----
> From: kbowden
> Date: Wednesday, October 8, 2008 5:37 pm
> Subject: Re: [Histonet] citrisolve?
>
> > I have used Citrisolve for serveral years. I don't think
> anything
> > compares to Xylene, but I do think this comes very close. I
> > change it
> > about once a week. It pretty much deparaffinizes in the same
> > amount of
> > time. Disposal is dependent on the regulations in your area.
> > When I
> > started using it our Environmental Health and Safety said pour
> it
> > down
> > the drain, but as time goes by and with more regulations now I
> > have to
> > collect it and have it removed with hazardous waste.
> > --
> > Karen Bowden
> > Staff Research Associate II
> > University of CA, San Diego
> > Department of Orthopedics
> > 9500 Gilman Dr. 0630
> > La Jolla, CA 92093-0630
> > 858-534-4655 voice
> > 858-534-5304 fax
> >
> >
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> >
> >
> > Patten, Nicole (NIH/NIAAA) [F] wrote:
> > > Does anyone have any experience using Citrisolve as a Xylene
> > alternative> to deparaffinize tissue sections? Do I use it just
> > like Xylene for the
> > > same amount of time? Can it be reused or should I use new
> Citrisolve> > each time? How do I dispose of it?
> > >
> > >
> > >
> > > Any help at all would be much appreciated. Thanks!
> > >
> > >
> > >
> > > Nicole J. Patten
> > > Post-Baccalaureate Fellow/IRTA
> > > National Institutes of Health
> > >
> > >
> > >
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>
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