Re: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa

From:renafail@bellsouth.net




 Are you filtering the solutions before immersing the slides?  The sodium chloride  is a  saturated solution.
 Rena Fail
 -------------- Original message from Jane M Flinn : --------------

We are running a congo red stain and have sodium chloride crystals precipataing 
> onto the slide. Does anyone know why and how we can fix this? jane
> 
> "Life is short - make haste to be kind"
> 
> Dr. Jane Flinn
> Director, Undergraduate Neuroscience Program
> George Mason University, 3F5
> 4400 University Dr.
> Fairfax, VA  22030
> Phone: 703-993-4107
> Fax: 703-993-1359
> 
> 
> ----- Original Message -----
> From: kbowden 
> Date: Wednesday, October 8, 2008 5:37 pm
> Subject: Re: [Histonet] citrisolve?
> 
> > I have used Citrisolve for serveral years.  I don't think anything 
> > compares to Xylene, but I do think this comes very close.  I 
> > change it 
> > about once a week.  It pretty much deparaffinizes in the same 
> > amount of 
> > time.  Disposal is dependent on the regulations in your area.  
> > When I 
> > started using it our Environmental Health and Safety said pour it 
> > down 
> > the drain, but as time goes by and with more regulations now I 
> > have to 
> > collect it and have it removed with hazardous waste.
> > --
> > Karen Bowden
> > Staff Research Associate II
> > University of CA, San Diego
> > Department of Orthopedics
> > 9500 Gilman Dr. 0630
> > La Jolla, CA 92093-0630
> > 858-534-4655 voice
> > 858-534-5304 fax
> > 
> > 
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> > 
> > Patten, Nicole (NIH/NIAAA) [F] wrote:
> > > Does anyone have any experience using Citrisolve as a Xylene 
> > alternative> to deparaffinize tissue sections? Do I use it just 
> > like Xylene for the
> > > same amount of time? Can it be reused or should I use new Citrisolve
> > > each time? How do I dispose of it? 
> > >
> > >  
> > >
> > > Any help at all would be much appreciated. Thanks!
> > >
> > >  
> > >
> > > Nicole J. Patten
> > > Post-Baccalaureate Fellow/IRTA
> > > National Institutes of Health
> > >
> > >  
> > >
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