Re: [Histonet] Microtome calibration

From:Rene J Buesa

That calibration is ussually done by measuring the advance mechanism of the block holder and how many Ám the block moves towards the blade, but that is rubbish.
RenÚ J.

--- On Fri, 10/31/08, Dr. med. Frauke Neff  wrote:

From: Dr. med. Frauke Neff 
Subject: Re: [Histonet] Microtome calibration
Date: Friday, October 31, 2008, 9:41 AM

Dear Dr. Girish,
I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if
 are 1Ám or 2.3Ám thick.
But I'm highly interested in how your GLP auditors want to calibrate the
thickness of the section cuts. Are you supposed to measure the slides after
cutting?! Before or after stretching in the water bath?! If they have a
protocol how to do this I would be happy if they can share it with us.


Quoting Rene J Buesa :

> Dr. Girish:
> BOTH "requirements" are rubbish invented by bureaucrats with
lots of time in
> their hands and trying to appear concerned and knowledgeable.
> Thickness is not necessary as long as the section is diagnostically useful
> if some quantitative method as to the intensity is done in which case
> thickness = amount of matter, and would influence the outcome of the
> quantitative process.
> Timing the tissue processor is also totally irrelevant because it does not
> really matter a few minutes each way during a processing protocol. More
> important would be to keep a record of the fixation time that is the only
> step really critical in tissue processing.
> RenÚ J.
> --- On Fri, 10/31/08,  wrote:
> From: 
> Subject: [Histonet] Microtome calibration
> To:
> Date: Friday, October 31, 2008, 2:49 AM
> Dear all
> Is anyone practising microtome calibration for thickness of sections cut?
> Our GLP auditors are frequently asking for it.
> They also suggest that automatic tissue processor time in each reagent
> should be calibrated.
> any comments
> Regards
> Dr Girish
> India
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