Re: [Histonet] MAOB IHC on either frozen or FFPE sections

From:Merced Leiker

I've never worked with MAOB, but what I do when I run into similar 
situations is to do a 1:50 and 1:200 dilution in parallel to start getting 
a feel for the antibody.  (Make sure to use antigen retrieval if you need 
it on the FFPE sections; apologies if you already knew that).

Yeah I, too, think it's weird that they illustrate the antibody at a 
particular concentration and then tell you they never determined the stock 


--On Friday, October 24, 2008 11:41 AM -0400 "Connolly, Brett M" 

> Has anyone done it? I have an antibody from Abnova. They illustrate
> staining of FFPE kidney at 3ug/mL, but when I asked what the stock Ab
> concentration is they say they never determined that. Go figure? I'm
> looking for a starting point dilution.
> Thanks,
> Brett
> Brett M. Connolly, Ph.D.
> Research Fellow, Imaging Research
> Merck & Co., Inc.
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Merced M Leiker
Research Technician II
354 BRB (packages) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
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