I've never worked with MAOB, but what I do when I run into similar 
situations is to do a 1:50 and 1:200 dilution in parallel to start getting 
a feel for the antibody.  (Make sure to use antigen retrieval if you need 
it on the FFPE sections; apologies if you already knew that).

Yeah I, too, think it's weird that they illustrate the antibody at a 
particular concentration and then tell you they never determined the stock 
concentration!

Merced

--On Friday, October 24, 2008 11:41 AM -0400 "Connolly, Brett M" 
 wrote:

> Has anyone done it? I have an antibody from Abnova. They illustrate
> staining of FFPE kidney at 3ug/mL, but when I asked what the stock Ab
> concentration is they say they never determined that. Go figure? I'm
> looking for a starting point dilution.
> Thanks,
> Brett
>
> Brett M. Connolly, Ph.D.
> Research Fellow, Imaging Research
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> PH 215-652-2501 fax. 215-993-6803
> e-mail. brett_connolly@merck.com
>
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Merced M Leiker
Research Technician II
354 BRB (packages) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

"Without my flaws I'm really very boring."
- random internet blog commentator


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