Amos:
One disadvantage for manual processing with IPA is a little
odor of "cat's urine". This is can fully eliminated when using VIP
processor.
We changed ethanol on IPA more than years ago and happy with it.
We also uses mineral oil instead xylene and also happy.
However none of our techs want not return to etanol, xylene and other
processing reagents.
If you want, please contact with me for details.
Rene very right.
Sincerely,
Maxim Peshkov
Russia,
Taganrog.

> --- On Fri, 10/3/08, Amos Brooks
>  wrote:
> 
> From: Amos Brooks 
> Subject: [Histonet] Isopropyl vs Ethanol
> To: "histonet@lists.utsouthwestern.edu"
> 
> Date: Friday, October 3, 2008, 8:31 AM
> 
> Hi,
>    Is there anyone who uses exclusively isopropyl
> alcohol to process with
> rather than ethanol? I have had great results with
> isopropyl alcohol for
> some tissues, but I wanted to know if there are any
> pitfalls to using it
> exclusively for processing. I've noticed that
> isopropyl is much cheaper
> than
> ethanol, so it leads me to wonder why ethanol is so
> prevalant for
> dehydration.
> 
> Thanks,
> Amos Brooks



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