Re: [Histonet] Heat and autofluorescence

From:Merced Leiker

I just realized - these are frozen sections!  I am getting my frozens and 
paraffins crossed here, sorry!  Don't heat them in that case - that would 
be disaster!


--On Thursday, October 09, 2008 8:16 PM +0000 wrote:

> I have a researcher in my lab that transfected cells with EGFP, injected
> them into mouse and then 3 weeks later harvested the tissue and frozen it
> in OCT as usual.  After sectioning, he air dried the slides for 1 hour
> and then dried them at 60 degrees C for 1 hour.  He was hoping just to
> see his EGFP infected cells which are bright green, however, the
> autofluorescence is everywhere - both with GFP and Rhodamine filters.
> Our question is - did the heating in the oven cause increased
> autofluorescence?
> Cathy
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Merced M Leiker
Research Technician II
354 BRB (Lee Lab) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

In order to put yourself in someone else's shoes,
you must first take off yours.

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