Re: [Histonet] Elastin Aldehyde Fuchsin

From:renafail@bellsouth.net




 The  solution does need to be filtered before each use
Rena Fail

  -------------- Original message from "Adam Boanas" : --------------


> Hello,
> 
>  
> 
> I have been using Gomori`s Aldehyde Fuchsin to stain human skin for
> elastin. The stain works fine and is clear and specific. I am using
> light green as a counterstain. My problem is that once stained, the
> slides have an excess granular purple "dotted" appearance upon the
> entire surface of the slide. This is not limited to the tissue position
> upon the slide, but across the clear glass too. We have tried using both
> superfrost plus charged slides and uncharged slides but the problem
> still is present. This precipitate is spoiling an otherwise great stain.
> Does anyone have any idea about what this is and how it could be
> prevented? (Our next guess would be to re-filter the fuchsin solution
> prior to use every time)
> 
>  
> 
> Many thanks
> 
> Adam
> 
>  
> 
> Adam Boanas 
> Histology Manager 
> Renovo Ltd 
> 
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