Re: [Histonet] Can you believe it

From:"louise renton"



Interestingly,
just yesterday I had a Tissue tek biopsy bag disintigrate in the Sakura
electrolytic decalcifier, but only the one nearest the (?) -ve terminal. I
thought those things were some sort of nylon and indestructible. Shows, you
live and learn!





On 11/25/08, stephanie.d.rivera@gsk.com  wrote:
>
> I can believe it!
>
> I have heard of the tissue disentergrating in decal(CAL-RITE) with heat.
> The stirrer was on and someone hit the heat knob and this was on a Friday.
> Well, on Monday there was no tissue, the cassette was chewed but it was
> there. Now we have tape across the heat knobs on all stirrer plates.
>
>
>
>
> Stephanie D. Rivera
>
> phone: 610-270-7340
>
>
>
>
> "Webb, Dorothy L" 
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 25-Nov-2008 16:02
>
> To
> histonet@lists.utsouthwestern.edu
> cc
>
> Subject
> [Histonet] Can you believe it
>
>
>
>
>
>
> Has anyone ever heard of cassettes and tissue totally disintegrating in
> formic acid decal solution with a high heat on the solution for about 3
> hours?????  We had 3 cassettes with tissue for decal placed in our
> container on the platform for adding agitation.  Someone had moved the
> knob to heat and this wasn't noticed until about 2-3 hours later, when
> it was boiling!  We cannot locate the cassettes (I think someone
> misplaced them) and others think they totally disintegrated with the
> decal and heat...what does everyone think?????  In all of my years of
> histology, never have I heard of anything so weird ......
>
> Dorothy Webb
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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
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