Re: [Histonet] Any experience with the Mohs "cryo embedder"

From:"Mark Tarango"



The Leica CM 1100 doesn't let you adjust the angle of the block face.  You
have to be flat.  I'll admit it's a major drawback.  I didn't even realize
it until you mentioned this.  I just got off the phone with leica and they
don't have anything to replace the specimen holder.  Looks like I'm just
stuck that way.


Mark

On Thu, Oct 9, 2008 at 2:49 PM, Mickie Johnson  wrote:

> Hi Mark,
>
> Thanks for your input on how the Leica CM 1100 is working for you.
> Regarding
> the best way to embed tissues for Mohs. Yes, the slide method is the most
> accurate to get the tissue flat as long as the epidermis is relaxed so it
> lays down on the glass too. Watch out for trapped air bubbles under the
> specimen. I smear a thin coat of O.C.T. on the slide and this helps hold
> the
> epi down during embedding. Does the Leica 1100 allow you to reposition the
> block face parallel to the knife when you embed? That is important so that
> you don't cut any more tissue away than necessary to get a full section of
> epidermis and deep margin. There is also a device which is very helpful in
> embedding Mohs specimens to minimize the need to line up the block face to
> the knife. Many labs use it. I carry one where ever I go to help me embed
> fast and accurately. If you would like to know more give me a call. Good
> Luck!
>
> Best Regards,
>
> Mickie
>
> Mickie Johnson, B.S., HTL(ASCP)
> Mohs Histology Consulting Services, LLC
>  & Mohs Lab Staffing
> 2507 S. Manito Blvd.
> Spokane, WA 99203
> 509-954-7134
> FAX   509-624-3926
> Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com
> Email: mickie25@netzero.net
>
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> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark
> Tarango
> Sent: Thursday, October 09, 2008 12:45 PM
> To: Ingles Claire
>  Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Any experience with the Mohs "cryo embedder"
>
> Does anyone else freeze the tissue to a glass slide to get it embedded
> flat?  That's how I've been show and it seems to work well.
>
> I've used plastic embedding molds too, but they don't always give me a flat
> surface.
> Mark
>
> On Thu, Oct 9, 2008 at 12:02 PM, Ingles Claire 
> wrote:
>
> > Sandra:
> > Who is selling this, and how does it work? I have been testing one type
> of
> > embedder, but I know there are several out there.
> > Claire
> >
> > ________________________________
> >
> > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Esparza,
> > Sandra
> > Sent: Thu 10/9/2008 11:20 AM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] Any experience with the Mohs "cryo embedder"
> >
> >
> >
> > Hi Everyone,
> >
> >
> >
> > I am new to the Mohs technique for dermatology specimens and am trying
> > to find out if anyone has used the Cryo Embedder system.  It sound great
> > but I don't want to order it until I'm sure that it is user friendly.
> > Any help with the Mohs technique would be greatly appreciated.   Thanks,
> >
> >
> > Sandra HT (ASCP)
> >
> > Dell Children's Hospital
> >
> > Austin, Texas
> >
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> >
> >
> >
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