Re: AW: [Histonet] Fatty tissue

From:"Angela Bitting"



We've done the same. Additional time in Xylene and paraffin made a huge
difference.

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
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>>> "Gudrun Lang"  11/6/2008 10:42 AM >>>
We have made some changes to our regular VIP-protocol (13 hours):
Increasing
time in 100%ethanol, in shell sol (=Xylen-substitute) and paraffin to
16
hours.
This led to an improvement of paraffin-infiltration and
sectioning-qualitiy.

Some blocks can be improved by letting them sit in melted paraffin for
a few
hours after the processing.
Gudrun

-----Urspr√ľngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von
Webb,
Dorothy L
Gesendet: Mittwoch, 05. November 2008 21:22
An: histonet@lists.utsouthwestern.edu 
Betreff: [Histonet] Fatty tissue

I would like to know how other labs are processing "fatty" tissue,
especially breast for optimal processing?  We have been doing a few
"reprocessing" of these specimens and my pathologist feels this should
not be happening!  Yes, the PA's are, at times, cutting the sections
larger than 3 mm thick, but, at times the tumor is ingrained in the
fatty area and they need to obtain as much of the perimeter as they
can.
Does anyone agitate or heat fixate their specimens prior to
processing?
Would appreciate any feedback on this age old dilemma.
Also, I only heard back from one person on the recycling issue.  Any
input would be appreciated as to savings incurred and a still vs.
recycler.
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