RE: [Histonet] re: Biocare Decloaker HIER

From:"Sebree Linda A."

I would say our "routine" HIER is 2 - 3 " in the decloaker after it gets
up to temp. and then a cool down equivalent to however long it takes for
the pressure to return to zero.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792

-----Original Message-----
[] On Behalf Of Jan
Sent: Tuesday, November 04, 2008 10:12 AM
To: histonet
Subject: [Histonet] re: Biocare Decloaker HIER

For those of you who use Biocare's Decloaker for HIER on regular cases
(not prion protein retrieval), could you tell me how long you do HIER
with these machines?  I normally do my prion cases in a Decloaker for
20', with a 25' cooldown, but I suspect that regular tissues don't need
that length of time at high pressure/temp.  I have an extra Decloaker
and am considering switching to this device for my normal case work
HIER, if it's time efficient.

Please reply privately.   Thanks.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
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