RE: [Histonet] experiences with kappa/lambda

From:"Dawson, Glen"


In my experience, Kappa & Lambda IHC staining tops my list of difficult antibodies.  Consistency was always poor & I've tried MANY of the available kappa/lambda antibodies out there (both polyclonal & monoclonal).  In situ hybridization is the way to go as I believe that IHC demonstration of these two antibodies will always be sub-standard.

My Opinion,
Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
[]On Behalf Of Gudrun
Sent: Wednesday, November 12, 2008 9:45 AM
Subject: [Histonet] experiences with kappa/lambda

Hi listmembers,

Please tell me about your experiences with the IHC-demonstration of kappa
and lambda on FFPE-sections.

We have some troubles about inconsistend staining and therefore false
results about the monoclonality of tumors.



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