Gudrun,

In my experience, Kappa & Lambda IHC staining tops my list of difficult antibodies.  Consistency was always poor & I've tried MANY of the available kappa/lambda antibodies out there (both polyclonal & monoclonal).  In situ hybridization is the way to go as I believe that IHC demonstration of these two antibodies will always be sub-standard.

My Opinion,
Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gudrun
Lang
Sent: Wednesday, November 12, 2008 9:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] experiences with kappa/lambda


Hi listmembers,

Please tell me about your experiences with the IHC-demonstration of kappa
and lambda on FFPE-sections.

We have some troubles about inconsistend staining and therefore false
results about the monoclonality of tumors.

Bye

Gudrun

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