RE: [Histonet] Plant Tissue Processing

From:"Alan Bright"



Dear Peggy,

I would have thought cryo sectioning would give much better results. 


Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com
Skype: dazzle0





-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peggy
Bisher
Sent: 12 November 2008 17:02
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Plant Tissue Processing

I have a professor here who would like me to make 10-15 micron thick
sections of lawn grass. I thought the best way to do this would be
paraffin
embedding. Does anybody have any suggestions as to the time/types for my
paraffin tissue processor? I am not very familiar working with plant
material.

Thank you very much, Maggie


Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
mbisher@princeton.edu




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